| 风信子品种间杂交与幼胚离体培养研究 |
| |
| 引用本文： | 李玉萍,王春彦,罗凤霞,韩美.风信子品种间杂交与幼胚离体培养研究[J].西北植物学报,2011,31(1). |
| |
| 作者姓名： | 李玉萍 王春彦 罗凤霞 韩美 |
| |
| 作者单位： | 金陵科技学院,园艺学院,南京,210038 |
| |
| 基金项目： | 国家948项目(2009-4-12); 金陵科技学院基金(JIT-RCYJ-2007001) |
| |
| 摘 要： | 选用5个风信子品种进行花粉生命力测定,对完全双列杂交获得的杂种种子剥胚进行离体培养.试验表明:(1)风信子不同品种花粉萌发率差别较大,其中Fondante的花粉萌发率最高为74.45%,Amsterdam的最低为12.68%,其他3个品种的花粉萌发率介于二者之间.(2)品种间自交杂交亲和力存在一定差异,结实率大于30%的组合从高到低依次为Fondante×Atlantic(61.2%)、Fondante×Fondante(54.0%)、Carnegie×Carnegie(42.6%)、JanBos×JanBos(36.0%)、Carnegie×Atlantic(32.7%)、Atlantic×Fondante(30.0%),组合Atlantic×Amster-dam、Carnegie×JanBos、Amsterdam×Fondante、Amsterdam×JanBos、JanBos×Amsterdam未得到果实,其余组合结实率介于0～30%之间.(3)适宜风信子杂交幼胚离体培养的初代培养基为MS+100 mg/L谷氨酰胺,其次为MS+0.2 mg/LIBA+0.02 mg/L6-BA+100 mg/L谷氨酰胺...
|
| 关 键 词： | 风信子 杂交组合 杂种胚 离体培养 |
Study on the Hybrid Hyacinthus orientalis Varieties and the Hybrid Embryo in vitro Culture |
| |
| LI Yu-ping,WANG Chun-yan,LUO Feng-xia,HAN Mei.Study on the Hybrid Hyacinthus orientalis Varieties and the Hybrid Embryo in vitro Culture[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(1). |
| |
| Authors: | LI Yu-ping WANG Chun-yan LUO Feng-xia HAN Mei |
| |
| Institution: | LI Yu-ping,WANG Chun-yan,LUO Feng-xia,HAN Mei(Jinling Institute of Technology,Nanjing 210038,China) |
| |
| Abstract: | 5 Hyacinthus orientalis varieties were selected to carry on the pollen vitality determination,the complete diallel crossing and culture the hybrid young embryo in vitro.The result showed:(1)A significant difference in pollen germination rate was found among various varieties,and the highest variety of pollen germination rate was Fondante(74.45%),and the lowest was Amsterdam,only 12.68%,and the other varieties were between the two;(2)Variety inbred line hybrid affinity existed certain difference.The combinat... |
| |
| Keywords: | Hyacinthus orientalis cross combination hybrid embryo in vitro culture |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
