| 内蒙古扁蓿豆遗传多样性的ISSR分析 |
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| 引用本文： | 李鸿雁,李志勇,王小丽,师文贵,邢建军.内蒙古扁蓿豆遗传多样性的ISSR分析[J].西北植物学报,2011,31(1):52-56. |
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| 作者姓名： | 李鸿雁 李志勇 王小丽 师文贵 邢建军 |
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| 作者单位： | 1. 中国农业科学院草原研究所,农业部沙尔沁牧草资源重点野外科学观测试验站,呼和浩特,010010
2. 中国农业科学院郑州果树研究所,郑州,450009 |
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| 基金项目： | 农业部农作物种质资源保护与利用项目(NB2010-2130135-32); 牧草种质资源保护项目(2010-43) |
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| 摘 要： | 应用ISSR分子标记对分布于内蒙古不同地区的扁蓿豆(Medicago ruthenica)8个地理种群进行了遗传多样性研究.结果表明:扁蓿豆具有较高的遗传多样性;15个引物共扩增出363个位点,物种水平上Nei s基因多样性指数为0.198 9,Shannon多样性指数为0.303 7;种内总基因多样性为0.308 6,种群内基因多样性为0.198 9,64.45%的遗传变异存在于种群内,35.55%的遗传变异存在于种群间,种群间的遗传分化系数为0.355 5,基因流为0.906 4,8个种群间基因交流较少,遗传分化较强.UPGMA遗传距离聚类结果表明,生态地理条件相似的种群优先聚集.
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| 关 键 词： | 扁蓿豆 ISSR 遗传多样性 Neis指数 Shannon指数 |
Genetic Diversity Studies of Medicago ruthenica in Inner Mongolia by ISSR |
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| LI Hong-yan,LI Zhi-yong,WANG Xiao-li,SHI Wen-gui,XING Jian-jun.Genetic Diversity Studies of Medicago ruthenica in Inner Mongolia by ISSR[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(1):52-56. |
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| Authors: | LI Hong-yan LI Zhi-yong WANG Xiao-li SHI Wen-gui XING Jian-jun |
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| Institution: | LI Hong-yan1,LI Zhi-yong1,WANG Xiao-li2,SHI Wen-gui1,XING Jian-jun1(1 Grassland Research Institute of the Chinese Academy of Agriculture Science,Shara Qin Key Wild Scientific Monitoring Station for Forage Resources of Ministry of Agriculture,Hohhot 010010,China,2 Zhengzhou Fruit Research Institute of the Chinese Academy of Agriculture Science,Zhengzhou 450009,China) |
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| Abstract: | The genetic diversity of Medicago ruthenica in the different regions of Inner Mongolia was analyzed by inter simple sequence(ISSR) markers.363 bands were amplified from 8 natural populations by 15 reliable primers.A high level of genetic diversity was revealed.The Nei's gene diversity index was 0.198 9 and the Shannon diversity index was 0.303 7.The total gene diversity was 0.308 6,while the gene diversity within population was 0.198 9,64.45% of genetic variation existed within population,while 35.55% of ge... |
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| Keywords: | Medicago ruthenica ISSR genetic diversity Nei's index Shannon index |
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