5种虾脊兰菌根显微结构观察

采用石蜡切片法观察5种虾脊兰菌根的显微结构、菌根真菌的侵入途径与分布特征,为更好地保护和开发利用虾脊兰属植物资源提供理论依据。结果表明:(1)5种虾脊兰菌根的显微结构由根被、皮层和中柱组成,根被细胞3～6层,皮层由9～13层薄壁细胞组成,韧皮部与木质部8～12束,呈辐射状相间排列,中柱中央为薄壁细胞组成的髓。(2)菌丝通过外皮层中的通道细胞侵入皮层,且皮层是内生菌根真菌寄居的主要区域,菌丝和菌丝结在靠近外皮层的几层皮层细胞中数量居多,菌丝在细胞核附近被消解,细胞核变形膨大。研究认为,5种虾脊兰可能拥有广泛的侵染能力较强的菌根真菌类型,使得这5种虾脊兰均具有较强的生态适应性。

Microscopic Observation on Mycorrhiza of Five Species of Calanthe

In order to provide a theoretical basis for better protection and utilization of the plant resources of Calanthe, we observed the microstructure, invasion pathway and distribution characteristics of mycorrhizal fungi roots of five species of Calanthe by paraffin section method. The results showed that: (1) the roots of five species of Calanthe were composed of the velamina, cortex and stele. The velamina consisted of 3-6 layers of cells, the cortex was composed of 9-13 layers of parenchymal cells, the phloem and xylem are 8-12 bundles, arranged radially, and the pith was located in the center of the stele. (2) The hyphae invades the cortex through passage cells in the exodermis. The cortex is the main colonization area of endomycorrhizal fungi, Most of the hyphae and hyphae were found in several layers of cortical cells near the exodermis. The hyphae is digested near the nucleus and the nucleus is deformed and expanded. According to the research, the five species of Calanthe may have a wide range of mycorrhizal fungal types, which make these five species of Calanthe have strong ecological adaptability.