阿魏酸和凹土对党参种子萌发、生长及幼苗叶绿素荧光参数的影响

该研究以党参种子为实验材料，通过设置不同浓度(0、0.01、0.05、0.2、0.5、1.0、2.0、3.0 、5.0和10.0 mmol·L^-1)的阿魏酸以及具有不同凹凸棒粘土(凹土)/蛭石体积比(0/1、1/120、1/80、1/50和1/20)基质的室内培育试验，分析基质中阿魏酸对党参种子萌发、生长及幼苗叶绿素荧光参数的影响，以及基质中凹土含量对上述阿魏酸影响的缓解效应。结果表明，当阿魏酸浓度高于1.0 mmol·L^-1时，党参种子活力受到抑制，种子萌发过程延缓。在播种前基质中施加不同浓度的阿魏酸，党参幼苗生物量积累和叶片光化学反应水平在低浓度下得到促进，在高浓度下受到抑制；在幼苗生长过程中短期内施加不同浓度的阿魏酸，党参幼苗叶片光化学反应水平会显著降低。在含有较高浓度阿魏酸的基质中添加不同体积比凹土，能够有效缓解上述党参种子萌发和幼苗生长过程中所遭受的阿魏酸伤害，且凹土在基质中的体积比为1/50时的缓解作用最为明显。可见，环境中高浓度阿魏酸会显著抑制党参种子萌发、生长及幼苗叶片光化学反应水平，添加适量凹土可有效缓解阿魏酸在不同生长阶段对党参所造成的胁迫伤害。

Effect of Ferulic Acid and Attapulgite on Seed Germination, Growth and Seedling Chlorophyll Fluorescence of Codonopsis pilosula

By using the seeds of Codonopsis pilosula as the experimental material and by cultivating C. pilosula with different concentrations (0, 0.01, 0.05, 0.2, 0.5, 1.0, 2.0, 3.0, 5.0 and 10.0 mmol·L^-1) of ferulic acid (FA) and the growth medium with different volume ratios of attapulgite clay (attapulgite)/vermiculite (0/1, 1/120, 1/80, 1/50 and 1/20) under indoor condition, we analyzed the effects of FA in the growth medium on the seed germination, growth, and chlorophyll fluorescence of C. pilosula, and studied whether the negative effects of FA on C. pilosula could be alleviated by different concentrations of attapulgite presented in the growth medium. The results showed that when the concentration of FA was higher than 1.0 mmol·L-1, the activity of C. pilosula seeds were inhibited and the germination of seeds was delayed. If FA was applied to the growth medium before cultivation, the biomass accumulation and photochemistry reaction of the C. pilosula seedlings were increased by lower concentrations of FA but were decreased by higher concentrations of FA. During the growth period of the seedlings, short term treatment with different concentrations of FA also significantly decreased the photochemistry reaction of the seedings. The attapulgite presented with different volume ratios in the growth medium can effectively alleviate the negative effects of higher concentration of FA on C. pilosula during the germination and growth. And, the volume ratio of attapulgite/medium at 1/50 seemed to be the most optimized content in alleviating these negative effects. These results indicate that high concentration of FA in environment can significantly inhibit the seed germination, growth, and photochemical reaction of C. pilosula seedling, while the addition of attapulgite into the medium with appropriate ratio can alleviate the damage of FA that would emerge at different stages during C. pilosula growth.