葡萄砧木耐盐性与丙二醛和脯氨酸关系的研究

采用水培法,对3个不同耐盐性葡萄砧木品种的1年生苗进行不同浓度的NaCl胁迫处理,研究叶片中丙二醛(MDA)和脯氨酸(Pro)含量的变化.结果表明,在NaCl胁迫下,不同耐盐性葡萄砧木品种的MDA和Pro含量变化不同.NaCl胁迫对高抗品种ZM01-1的MDA含量影响较小,MDA含量处于一个比较稳定的水平,而其它品种的MDA变化幅度大;NaCl胁迫下Pro含量呈波动式变化,在0.1%NaCl胁迫下高抗品种ZM01-1的Pro积累少,0.3%NaCl下ZM01-1的Pro积累多.研究认为可以把MDA含量的相对稳定作为葡萄耐盐性鉴定的辅助指标,而Pro不宜作为葡萄耐盐性鉴定指标.

Relation between Salt Tolerance of Grape Rootstock and MDA and Proline Contents in Grape Leaves

One-year-old seedling of three rootstock varieties of grape with different salt tolerances,which were hydroponically cultured,were treated with NaCl at different concentrations to examine how the MDA and proline contents varied in the leaves.It was shown that under salt stress,the rootstock varieties of grape with different salt tolerances differed in their leaves MDA and proline content.Salt stress affected the MDA content in the leaves of ZM01-1,a variety with high salt tolerance,to a lower extent and the MDA content remained at a stable level,but caused the MDA contents in the leaves of the other varieties to vary to a large extent.Under salt stress the proline contents appeared to vary in a wavy manner.ZM01-1,a variety with high salt tolerance,accumulated a small amount of proline at 0.1% NaCl and a big amount of proline at 0.3% NaCl.The study indicated that MDA content could be used as an auxiliary index to evaluate salt tolerances of grape because of its relative stability and proline content were not suitable to be used in salt tolerance evaluation of grape.