小麦苗期冷驯化相关cDNA的克隆

以中国春小麦幼苗为材料,利用改进的mRNA差异显示方法和银染技术,对不同低温驯化诱导表达的差异基因进行分离,共得到cDNA差异片段60条.经Reverse Northern验证,检出阳性表达片段8条,克隆并测序,分别命名为TIGW1~TIGW8.其中,TIGW2和TIGW3可能与抗冻蛋白积累、低温诱导蛋白的生成或低温信号的感受与传递有关;TIGW1、TIGW6、TIGW8中具有ACGT应答元件,TIGW3含有CAACA应答元件,说明可能获得了冷诱导基因的启动子序列.

Cloning of Related cDNAs Induced under Cold Acclimation in Wheat Seedling Stage

Both mRNA differential display and silver staining techniques were used to isolate Chinese Spring cDNAs induced by low temperature acclimation in wheat seedling stage.A total of 60 differential expressed cDNA fragments were obtained.Eight positive cDNA fragments,called TIGW1 to TIGW8,have been cloned and sequenced after testifying by Reverse Northern.TIGW2 and TIGW3 are probably related to the accumulation of antifreezing proteins,the formation of low temperature induced proteins or the reception and transfer of low temperature signal;TIGW1,TIGW6 and TIGW8 have ACGT responsive elements,TIGW3 has CAACA responsive element,so we may obtain the promoter sequence of cold induced genes.