| 青钱柳组培快繁体系的初步研究 |
| |
| 引用本文： | 谢寅峰,王莹,尚旭岚,杨万霞,方升佐.青钱柳组培快繁体系的初步研究[J].西北植物学报,2009,29(11). |
| |
| 作者姓名： | 谢寅峰 王莹 尚旭岚 杨万霞 方升佐 |
| |
| 作者单位： | 南京林业大学,森林资源与环境学院,南京,210037 |
| |
| 基金项目： | 江苏省高等学校创新团队科研计划，江苏省"六大人才高峰"资助项目，江苏省高新技术研究计划 |
| |
| 摘 要： | 以青钱柳成熟胚为实验材料,对建立青钱柳组培快繁技术体系进行初步研究.结果表明,离体胚在添加30 g/L蔗糖、6.5 g/L琼脂的培养基中培养60 d,成苗率可达到72.67%.通过正交试验筛选出丛生芽诱导增殖的最佳培养基为WPM+0.5 mg/L 6-BA+0.01 mg/L IBA+30 g/L蔗糖,适宜的继代周期为40 d,丛生芽诱导率可达到93.33%,增殖系数最高为4.75,3种激素对丛生芽增殖系数的影响程度依次为IBA>6-BA>KT,其中IBA有显著影响.培养基中添加适当浓度的烯效唑和稀土镧对试管苗的壮苗和生长均有促进作用,其最适浓度分别为0.1 mg/L和5 mg/L.适宜浓度的IBA对试管苗生根有一定促进作用,采用WPM+0.2 mg/L IBA+20 g/L蔗糖培养基进行诱导,生根率为16.67%,经过15 d暗诱导处理,生根率可提高到23.33%.
|
| 关 键 词： | 青钱柳 组织培养 丛生芽 诱导增殖 壮苗 生根 |
Preliminary Study on the Tissue Culture and Rapid Propagation System of Cyclocarya paliurus |
| |
| XIE Yin-feng,WANG Ying,SHANG Xu-lan,YANG Wan-xia,FANG Sheng-zuo.Preliminary Study on the Tissue Culture and Rapid Propagation System of Cyclocarya paliurus[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(11). |
| |
| Authors: | XIE Yin-feng WANG Ying SHANG Xu-lan YANG Wan-xia FANG Sheng-zuo |
| |
| Institution: | XIE Yin-feng,WANG Ying,SHANG Xu-lan,YANG Wan-xia,FANG Sheng-zuo (College of Forest Resources , Environment,Nanjing Forestry University,Nanjing 210037,China) |
| |
| Abstract: | Using mature embryo of Cyclocarya paliurus as experimental materials,we preliminarily established the tissue culture and rapid propagation system in this experiment.The result indicated that the seeding rate of excised embryos was higher in culture medium with 30 g/L sucrose and 6.5 g/L agar for 60 days,which reached 72.67%.Through orthogonal test,the optimal culture medium for multiple shoots induction was composed of WPM+0.5 mg/L 6-BA+0.01 mg/L IBA+30 g/L sucrose and the suitable subculture cycle was 40 d... |
| |
| Keywords: | Cyclocarya paliurus tissue culture clustered shoots induction and proliferation strong seedling rooting |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
