| 膜荚黄芪种子中2种几丁质酶的分离纯化及活性测定 |
| |
| 引用本文： | 费凡,闫巧娟,江正强,Kumar Narasimha.膜荚黄芪种子中2种几丁质酶的分离纯化及活性测定[J].西北植物学报,2009,29(12). |
| |
| 作者姓名： | 费凡 闫巧娟 江正强 Kumar Narasimha |
| |
| 作者单位： | 1. 中国农业大学,工学院,北京,100083
2. 中国农业大学,食品科学与营养工程学院,北京,100083 |
| |
| 基金项目： | 国家自然科学基金，教育部"新世纪优秀人才支持计划" |
| |
| 摘 要： | 运用传统的层析技术对膜荚黄芪种子中两种几丁质酶进行分离纯化,并对分离纯化中各个步骤得到的蛋白进行了活性研究.结果表明:(1)粗提液的硫酸铵沉淀经再生几丁质亲和柱和凝胶过滤层析Sephadex G-75得到几丁质酶A.(2)粗提液的硫酸铵沉淀经离子交换色谱DE-52、CM、凝胶过滤层析Sephadex G-75得到几丁质酶B.(3)几丁质酶A、B的比活性分别为35.6 U/mg和4.1 U/mg.(4)从膜荚黄芪种子中分离纯化的几丁质酶A和B均为糖蛋白,含糖量分别为6.1%和5.8%.(5) SDS-PAGE显示,几丁质酶A、B的分子量分别为35.5 ku、39.6 ku;凝胶过滤层析测定几丁质酶A、B的分子量分别为36.9 ku、40.8 ku;表明几丁质酶A、B均为单亚基蛋白.
|
| 关 键 词： | 黄芪种子 几丁质酶 纯化 亲和层析 |
Purification of Two Chitinases from the Seeds of Astragalus membranaceus(Fisch.) Bge.and Determination of Their Activities |
| |
| FEI Fan,YAN Qiao-juan,JIANG Zheng-qiang,Kumar Narasimha.Purification of Two Chitinases from the Seeds of Astragalus membranaceus(Fisch.) Bge.and Determination of Their Activities[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(12). |
| |
| Authors: | FEI Fan YAN Qiao-juan JIANG Zheng-qiang Kumar Narasimha |
| |
| Institution: | FEI Fan1,YAN Qiao-juan1,JIANG Zheng-qiang2,Kumar Narasimha2(1 College of Engineering,China Agricultural University,Beijing 100083,China,2 College of Food Science , Nutritional Engineering,China) |
| |
| Abstract: | Two chitinases were purified from the seeds of Astragalus membranaceus(Fisch.) Bge.using traditional chromatographies and the activities of the proteins obtained during each purification step was determined.Results showed:(1)a chitinase(designated as chitinase A) was purified using a combination of 20-60 % ammonium sulfate saturation,regenerated chitin column and Sephadex G-75.(2)Another chitinase(designated as chitinase B) was purified by a procedure containing ammonium sulfate precipitation,ion-exchange c... |
| |
| Keywords: | Astragalus membranaceus (Fisch ) Bge seeds chitinase purification affinity chromatography |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
