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菘蓝不同器官脂溶性成分的GC-MS分析
引用本文:王文杰,罗光明,李焘,王喆之.菘蓝不同器官脂溶性成分的GC-MS分析[J].西北植物学报,2011,31(4):823-828.
作者姓名:王文杰  罗光明  李焘  王喆之
作者单位:1. 陕西师范大学,药用资源与天然药物化学教育部重点实验室/西北濒危药材资源开发国家工程实验室,西安,710062;江西中医学院江西省中药种质资源工程技术研究中心,南昌,330004
2. 江西中医学院江西省中药种质资源工程技术研究中心,南昌,330004
3. 陕西师范大学,药用资源与天然药物化学教育部重点实验室/西北濒危药材资源开发国家工程实验室,西安,710062
摘    要:采用索氏提取法对菘蓝根、茎、叶、花和幼果等不同器官的脂溶性成分进行提取,利用气相色谱-质谱联用(GC-MS)技术对其脂溶性成分进行鉴定,并运用面积归一化法确定相对含量,以明确菘蓝的化学物质基础,为综合开发利用菘蓝资源提供依据。结果显示:从菘蓝的根、茎、叶、花和幼果中分别鉴定出24、23、21、18和23种脂溶性成分;其中,十二烷、十三烷、十四烷、2,4-二叔丁基苯酚和棕榈酸为各器官的共有成分;不同器官中脂肪酸含量依次为:花(80.91%)>根(60.56%)>幼果(21.93%)>茎(17.45%)>叶(11.28%)。除花中的饱和脂肪酸含量高于不饱和脂肪酸外,其他器官中不饱和脂肪酸的含量均较高,表明菘蓝不同器官中脂溶性成分的组成及含量存在一定差异。

关 键 词:菘蓝  脂溶性成分  索式提取  GC-MS

GC-MS Analysis and Comparison of Fat-soluble Components of Different Organs from Isatis indigotica
WANG Wen-jie,LUO Guang-ming,LI Tao,WANG Zhe-zhi.GC-MS Analysis and Comparison of Fat-soluble Components of Different Organs from Isatis indigotica[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(4):823-828.
Authors:WANG Wen-jie  LUO Guang-ming  LI Tao  WANG Zhe-zhi
Institution:WANG Wen-jie1,2,LUO Guang-ming2,LI Tao1,WANG Zhe-zhi1(1 Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry of Ministry of Education,National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drug in Northwest of China,Shaanxi Normal University,Xi'an 710062,China,2 The Chinese Medicine Germplasm Resource Engineering Technology Research Center of Jiangxi Province,Nanchang 330004,China)
Abstract:The fat-soluble components of different organs from Isatis indigotica Fort.were extracted by Soxlet extraction methods,and analyzed and identified by GC-MS.There are 24,23,21,18 and 23 kinds of components were identified from radix,stem,leaf,flower and fruitlet,respectively,of which included 5 kinds of common compounds,such as dodecane,tridecane,tetradecane,2,4-bis(1,1-dimethylethyl)-phenol and hexadecanoic acid.The relative contents of fatty acid from different organs were in the following order:flower(80....
Keywords:GC-MS
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