| 一株产铁载体内生细菌对尖孢镰刀菌的拮抗作用 |
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| 引用本文： | 韩松,张守村,黄晓艳,林天兴,龚明福.一株产铁载体内生细菌对尖孢镰刀菌的拮抗作用[J].西北植物学报,2011,31(5):1039-1044. |
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| 作者姓名： | 韩松 张守村 黄晓艳 林天兴 龚明福 |
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| 作者单位： | 1. 塔里木盆地生物资源保护利用兵团重点实验室,塔里木大学生命科学学院,新疆阿拉尔,843300
2. 乐山师范学院化学与生命学院,四川乐山,634004 |
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| 基金项目： | 国家自然科学基金，塔里木大学校长基金重点项目 |
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| 摘 要： | 通过改良蔗糖-天冬氨酸培养基筛选到一株产铁载体的内生细菌HS-4,测定了该菌在不同铁离子浓度下对棉花枯萎病致病菌尖孢镰刀菌(Fusarium oxysporum)的抑菌效果,并结合形态、生理生化、16S rDNA序列同源性和系统发育分析对菌株进行鉴定.结果表明:内生细菌HS-4在MSA培养基中产生荧光型铁载体,其铁载体相对含量为80%.该铁载体在低铁条件下对F.oxysporum具有抑制作用.内生细菌HS-4初步鉴定为萎缩芽孢杆菌(Ba-cillus atrophaeus).
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| 关 键 词： | 内生细菌 铁载体 尖孢镰刀菌 萎缩芽孢杆菌 |
Antagonism of a Siderophore-producing Entophytic Bacteria on Fusarium oxysporum |
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| HAN Song,ZHANG Shou-cun,HUANG Xiao-yan,LIN Tian-xing,GONG Ming-fu.Antagonism of a Siderophore-producing Entophytic Bacteria on Fusarium oxysporum[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(5):1039-1044. |
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| Authors: | HAN Song ZHANG Shou-cun HUANG Xiao-yan LIN Tian-xing GONG Ming-fu |
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| Institution: | HAN Song1,ZHANG Shou-cun1,HUANG Xiao-yan1,LIN Tian-xing2,GONG Ming-fu1(1 Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin of Xinjiang,Production Construction Corps,College of Life Science,Tarim University,Alar,Xinjiang 843300,China,2 School of Chemistry and Life Sciences,Leshan Normal University,Leshan,Sichuan 571101,China) |
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| Abstract: | In this study,a siderophore-producing endophytic strain was isolated using the improved sugar-aspartic acid(MSA) as selective medium,and then detected its antibacterial effect against fusarium wilt pathogens(Fusarium oxysporum) at different concentrations of FeCl3.This strain was identified by using morphological,biochemical and physiological characteristics,16S rDNA sequence homology and phylogenetics analysis.The results showed that strain HS-4 produced the fluorescent siderophores(pyoverdine) on MSA medi... |
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| Keywords: | endophytic bacteria siderophore Fusarium oxysporum Bacillus atrophaeus |
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