马铃薯杂种F1的SSR鉴定

为选育抗黑痣病、高产优质的马铃薯新品种,选用引进品种‘大西洋’分别与‘陇薯6号’、‘陇薯7号’杂交,获得了杂种F1代,利用SSR标记技术对‘大西洋’与‘陇薯6号’的42个杂种F1、‘大西洋’与‘陇薯7号’的9个杂种F1单株进行了鉴定。从59对SSR引物中筛选出2对在亲本间存在差异、扩增稳定、条带清晰的引物S184和STM1049,用于‘大西洋’ב陇薯6号’杂种F1、‘大西洋’ב陇薯7号’杂种F1及其亲本的基因组DNA扩增。SSR带型分析显示,杂种F1的SSR带型呈双亲互补型、缺失型、父本型和母本型4类,依据带型特征鉴定出供试的51个马铃薯杂种F1单株均为真杂种,表明SSR分子标记技术用于马铃薯杂种真实性鉴定是可行的。该研究可为进一步开展马铃薯杂交后代目标性状优异株系选育提供依据。

Identification of Solanum tuberosum Hybrids by SSR Molecular Markers

In order to breeding new potato(Solanum tuberosum) varieties with anti-Rhizoctonia solani,high yield and good quality,’Longshu No.6’ and ’Longshu No.7’ was crossed with ’Atlantic’,respectively,and the F1 hybrids were obtained.42 F1 hybrid individuals of ’Atlantic’×’Longshu No.6’,and 9 F1 hybrid individuals of ’Atlantic’×’Longshu No.7’ were used for analysis with SSR molecular markers.Two pairs of SSR primers were selected from 59 pairs of SSR primers,and were used for marker analysis among those hybrids and their parents.The results showed that there were 4 types of banding patterns observed in the hybrids,complementary pattern of both parents,deletion pattern,male-specific pattern and female-specific pattern.Fifty one individuals of F1 hybrids were identified as true hybrids with primers S184 and STM1049,which showed that it was feasible to identify hybrid potatoes with SSR molecular markers.This research may provide the theoretical basis for further selection of excellent lines with objective traits from potato hybrid descendants.