| 四倍体青花菜低稔性胚胎学研究 |
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| 引用本文： | 李俊星,张蜀宁,赵冬,刘惠吉.四倍体青花菜低稔性胚胎学研究[J].西北植物学报,2009,29(11). |
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| 作者姓名： | 李俊星 张蜀宁 赵冬 刘惠吉 |
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| 作者单位： | 南京农业大学,园艺学院,南京,210095;农业部南方蔬菜遗传改良重点开放实验室,南京,210095 |
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| 基金项目： | 江苏省自然科学基金项目 |
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| 摘 要： | 以二、四倍体青花菜为材料,在现蕾期分别套袋自交,对四倍体与二倍体的胚囊结构、受精以及受精后胚胎发育过程进行比较研究.结果表明:四倍体青花菜胚囊发育完整,与二倍体相似.四倍体花粉萌发率较二倍体低,花粉管生长较慢且不整齐.四倍体和二倍体青花菜自交均能正常受精,四倍体青花菜在受精后胚胎发育的进程上均较其二倍体滞后约2 d,且胚胎发育在进度上不一致,并存在胚胎败育现象,正常胚胎只占25.8%,导致四倍体结实率比二倍体降低22.6%.
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| 关 键 词： | 青花菜 同源四倍体 二倍体 受精率 胚胎发育 |
Mechanism of Low Fertility in Autotetraploid Broccoli |
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| LI Jun-xing,ZHANG Shu-ning,ZHAO Dong,LIU Hui-ji.Mechanism of Low Fertility in Autotetraploid Broccoli[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(11). |
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| Authors: | LI Jun-xing ZHANG Shu-ning ZHAO Dong LIU Hui-ji |
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| Institution: | LI Jun-xing1,2,ZHANG Shu-ning1,2*,ZHAO Dong1,LIU Hui-ji1,2 (1 College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China,2 Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,China) |
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| Abstract: | Embryo sac structure,fertilization process and embryo development of the diploid and autotetraploid broccoli were studied after self-pollinated in bud stage.The results indicated that autotetraploid broccoli embryo sac development was normal which was similar with diploid.The frequency of pollen germination of autotetraploid broccoli was lower and the pollen tubes grew slowly and nonuniformly.Both diploid and autotetraploid broccoli gave normal fertilization.Compared with diploids,autotetraploid Embryonic d... |
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| Keywords: | broccoli autotetraploid diploid fertilization rate embryonic development |
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