亚麻遗传转化体系的建立及几丁质酶基因导入的研究

报道了亚麻遗传转化体系的建立和几丁质酶基因对亚麻遗传转化的研究。亚麻下胚轴切段培养在不同激素浓度的ＭＳ培养基上，诱导分化出不定芽。最佳的激素组合是ＭＳ＋ＢＡ１ｍｇ／Ｌ＋ＩＡＡ０．５ｍｇ／Ｌ，分化频率可达９７％。亚麻的下胚轴经带有几丁质 根癌农杆菌感染后，在含有１００ｍｇ／Ｌ卡那霉素的选择分化培养基上，１４￣２１ｄ就能产生抗生小芽，小芽进一步伸长后可在１００ｍｇ／Ｌ卡那霉素的ＭＳ选择生根培养基（ＭＳ

Transformations system and study on the introduction of chitinase gene into Linum usitatissium

Optimal shoot differentiation from hypocotyl explants of Linum usitatissium was MS medium supplemented with BA 1 mg/L and IAA 0.5 mg/L,which resulted in 97% of shoot regeneration frequency.The hypocotyl explants were infected by Agrobacterium tumefaciens containing chitinase gene,and subcultured on MS selection medium containing kanamycin 100 mg/L to select transformants.Then,the transformed shoots were rooted.Rooting selected and the leaf callus assay indicated that the chitinase gene had been introduced into genome of Linum usitatissium .