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两个小麦磷转运蛋白基因的分离、功能鉴定和表达研究
引用本文:常胜合,舒海燕,童一平,秦广雍,李滨,李振声.两个小麦磷转运蛋白基因的分离、功能鉴定和表达研究[J].西北植物学报,2004,24(10):1779-1785.
作者姓名:常胜合  舒海燕  童一平  秦广雍  李滨  李振声
作者单位:1. 中国科学院遗传与发育生物学研究所,北京,100101;郑州大学,离子束生物工程实验室,郑州,450056
2. 郑州大学,生物工程系,郑州,450001
3. 中国科学院生态环境研究中心,北京,100085
4. 郑州大学,离子束生物工程实验室,郑州,450056
5. 中国科学院遗传与发育生物学研究所,北京,100101
基金项目:国家重大基础研究与发展规划项目(G1998010200)
摘    要:磷是能量代谢、核酸以及许多生物膜合成的重要底物。在光合作用、呼吸作用等过程中发挥了重要作用。中国大多数小麦产区的土壤存在着缺磷的问题。磷饥饿给小麦生产造成了很大损失。培育耐低磷小麦是解决这一问题的一个重要途径。在磷饥饿的过程中,哪些基因的表达发生了变化.它们是如何变化的,弄清楚这些问题对于培育转基因耐低磷小麦具有重要的意义。磷转运蛋白基因在植物吸收磷的过程中发挥着重要作用。利用RT—PCR的方法,我们从普通小麦“小偃54”中分离了两个磷转运蛋白基因TaPT8和TaPHT2;1。通过与酵母突变体互补分析表明这两个基因都能够与磷吸收功能存在缺陷的酵母突变体实现功能互补,在低磷条件下有促进酵母突变体吸收磷的作用。进一步分析表明TaPT8属于Pht1家族。TaPHT2;1属于Pht2家族。运用RQRT—PCR的方法进行分析后发现TaPT8在根中表达,受磷饥饿的诱导;TaPHT2;1主要在绿色组织中表达,受磷饥饿的抑制,受光的诱导。TaPT8可能主要参与了小麦的根从土壤中吸收磷的过程。TaPHT2;1可能在磷从细胞质向叶绿体内转运的过程中发挥了重要作用。

关 键 词:磷饥饿  普通小麦(Triticum  aestivum  L.)  磷转运蛋白  功能

Isolation,function and expression analysis of two wheat phosphate transporter genes
Abstract.Isolation,function and expression analysis of two wheat phosphate transporter genes[J].Acta Botanica Boreali-Occidentalia Sinica,2004,24(10):1779-1785.
Authors:Abstract
Abstract:Phosphate (Pi) is one of the key substrate in energy metabolism and biosynthesis of nucleic acids and membranes.It also plays an important role in photosynthesis,respiration,and regulation of a number of enzymes.Most of the areas of wheat growth in China face the problem of phosphate deficiency.Generating varieties that can grow well under the condition of phosphate starvation is an important way to solve this problem.What genes expression changed when wheat grew under the condition of phosphate starvation?How did they change?Solving these problems is very important for generating transgenic wheat varieties that can produce adequate yields in phosphate-deficiency soils.Phosphate transporters were one important family of these genes.Using the known ESTs BQ744323(5'),BJ287203(3'),BJ260111(5') and BJ265811(3'),two phosphate transporter gene cDNAs TaPT8 and TaPHT2;1 were isolated from common wheat by RT-PCR.The two phosphate transporter genes can complement the phosphate-deficiency in the yeast mutants.TaPT8 belonged to Pht1 family.TaPHT2;1 is one member of Pht2 family.Using RQRT-PCR,the expression of TaPT8 could only be found in roots and was up-regulated by phosphate starvation.TaPHT2;1 mainly expressed in green tissues.The expression of TaPHT2;1 was down-regulated by phosphate starvation and darkness but up-regulated by light using RQRT-PCR.TaPHT2;1 may play a role during the process of transport phosphate from cytoplasm into chloroplast.
Keywords:phosphate starvation  common wheat ( Triticum aestivum  L  )  phosphate transporter  function
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