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红豆草体细胞胚胎发生早期过氧化物酶和酯酶同工酶酶谱变化
引用本文:路铁刚,王义琛,郑国錩.红豆草体细胞胚胎发生早期过氧化物酶和酯酶同工酶酶谱变化[J].西北植物学报,1990(1).
作者姓名:路铁刚  王义琛  郑国錩
作者单位:中国科学院植物研究所,兰州大学细胞生物学研究室,兰州大学细胞生物学研究室 兰州大学细胞生物学研究室兰州 730000,兰州 730000,兰州 730000
摘    要:红豆草下胚轴切段接种于含1mg/l BA,1mg/l KT的LS培养基上,通过筛选和繁殖由一块外植体而来的淡黄色愈伤组织,而得到生理状态比较一致具有较高胚性发生能力的非胚性愈伤组织,将其转移到含1mg/l BA的LS培养基上后可诱导体细胞胚眙发生。在体细胞胚胎发生早期发现过氧化物酶同工酶和酯酶同工酶酶谱均有规律性变化。过氧化物酶同工酶酶谱在胚性培养的第10天,两条明显的A_1、A_2带消失。酯酶同工酶各酶带之间酶活性比例在胚性培养过程中变化很大,培养后期酶带变得不明显或酶带数下降。说明胚性发生过程遗传信息的表达有选择性并为激素所调控。

关 键 词:红豆草  体细胞胚胎发生  过氧化物酶  酯酶  同工酶

CHANGES OF ISOENZYME PATTERNS OF PEROXIDASE AND EASTERASE DURING EARLY PROCESS OF SOMATIC EMBRYOGENESIS IN SAINFOIN (Onobrychis viciaefolia Scop)
Lu Tie-gang,Wang Yi-shen and Zheng Guo-chang.CHANGES OF ISOENZYME PATTERNS OF PEROXIDASE AND EASTERASE DURING EARLY PROCESS OF SOMATIC EMBRYOGENESIS IN SAINFOIN (Onobrychis viciaefolia Scop)[J].Acta Botanica Boreali-Occidentalia Sinica,1990(1).
Authors:Lu Tie-gang  Wang Yi-shen and Zheng Guo-chang
Abstract:Hypocotylar explants of Onobrychts viciaefolia Scop were cultured on LS medium containing 1 mg/l BA, 1 mg/l KT. Two weeks after culture calli were initiated on the surface of sections. Nonembryogenic callus but with simitiar physiological state and higher embryogenic capacity was obtained by the selection and proliferation of light-yellow callus formed from one explant, and then it was trans ferred to LS medium containing 1mg/l BA to initiate somatic embryogenesis. Obvious changes of isoenzyme patterns were found. Two marked bands of isoperoxidase disappeared 10 days after embryogenic culture. The activity ratio of each isoeasterase band changed greatly during somatic embryogenesis, the number ofisoeasterase bands reduced during later embryogenie culture. Some isoenzyme bands appeared or became stronger while others disappeared or became weaker during somatic embryogenesis. From the results the author suggested that the expressions of venetic informations had a selective mechanism and were regulated by hormones.
Keywords:Sainfoin  Somatic embryogenesis  Peroxidase  Easterase  Isoenzyme  
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