黑麦花粉母细胞的分离与培养及其细胞融合现象的观察

ISOLATION, CULTURE AND OBSERVATION OF CYTOMIXIS, OF MICROSPOROCYTES OF RYE

The microsporocytes, being in early prophase of meiosis I, of rye(Secale cereal L.) were isolated out of their anther sacs as a filament,cultured in a microchamber and observated directly under the micros-cope. In this condition that the microsporocytes kept intact and livingstate, cytomixis activity still existed and showed the same ways asthat seen in the preparations of squashing and section did. Under thecondition of the liquid suspension culture of microsporocyte filament,the microsporocytes were able to continue developing and part of microsporocytes became abnormal in the meiotic behavior. The abnor-mal behavior mainly included an increase and a decrese of chromatinand chromosome number. This study futher proved our early conclu-sions that the action of machnical injuries and fixatives is not the cau-se of cytomixis and that cytomixis might induce the genome of somecells to change.