印度块菌子囊果内部解剖结构的扫描电镜观察

利用扫描电镜对成熟印度块菌子囊果的内部结构进行了观察,以系统揭示其子囊果内部组织特征,为块菌属的分类以及块菌属真菌子囊果的生理研究奠定基础。观察结果进一步证明,成熟印度块菌子囊果横切面上的白色迷宫状脉络是由不育的侧丝构成,而暗脉则是被侧丝缠绕并包裹着的可育的菌丝组织,即产孢组织,这些白色脉络和暗脉就构成了印度块菌子囊果横切面上迷宫状的纹脉;产孢组织中,可观察到正在发育的大大小小的子囊被缠绕在一起的大量产囊丝与侧丝包裹着,形成密密麻麻微小的类似蜂巢状结构;子囊孢子游离于子囊中,成熟子囊孢子表面有刺状纹饰,刺的顶端有小弯钩。单个子囊内含的子囊孢子大小与其内含的子囊孢子数目有关,子囊内所含的子囊孢子越多,子囊孢子就越小。

Scanning Electron Microscope Observation on Anatomical Structure of Tuber indicum Ascocarps

The internal structure of mature Tuber indicum ascocarps were observed by means of scanning electron microscope. The result further confirmed that, the white veins covered on the cross section of mature T. indicum ascocarps were composed of sterile paraphysis, while the dark veins which were to be the gleba were the fertile hyphostroma intertwined and surrounded by paraphysis, these white and dark veins constituted the maze veins on the cross section of T. indicum ascocarps; the developing uniform asci surrounded by plenty of intertwined ascogenous hypha and paraphysis could be able to be observed in the gleba, forming a thickly dotted tiny honeycomb like structure; the ascospores distributed free in asci, densely ornamented with stings on the epispore of fully mature spores, slightly having a crook on the tip of sting. The size of ascospores in an ascus was related to the number of ascospores contained in this ascus, the more ascospores contained in an ascus, the smaller the ascospores would be. The internal anatomy structure observation of T. indicum ascocarps by means of scanning electron microscope in great detail can provide reliable and visualized accordance for classifying of Tuber and understanding the internal characteristics of Tuber ascocarps systematically, as well as forming the foundation for the psychological study of Tuber ascocarps.