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珍稀濒危植物蒙古扁桃的组织培养及植株再生
引用本文:斯琴巴特尔,满良,等.珍稀濒危植物蒙古扁桃的组织培养及植株再生[J].西北植物学报,2002,22(6):1479-1481.
作者姓名:斯琴巴特尔  满良
作者单位:内蒙古师范大学生物系,呼和浩特,010022
基金项目:内蒙古自治区自然科学基金资助项目 ( 980 2 0 2 ),内蒙古教育厅科研基金资助项目 ( ZD980 70 2 )
摘    要:对珍稀濒危植物蒙古扁桃进行组织培养获得再生植株。实验结果表明,在MS培养基上蒙古扁桃幼苗茎尖,茎切段和叶片等外植体均可以脱分化形成愈伤组织,并进一步分化形成再生植株。器官的脱分化与再分化决定于培养基中的激素种类及其浓度。诱导愈伤组织形成的最适培养基为MS+6-BA0.8mg/L NAA0.1mg/L,芽分化诱导最适培养基为MS+6-BA0.8mg/L,诱导生根的最适培养基是MS+IBA0.5mg/L。

关 键 词:珍稀濒危植物  蒙古扁桃  组织培养  植株再生
文章编号:1000-4025(2002)06-1479-03
修稿时间:2001年12月25

Tissue culture and plantlet regeneration of endangered rare plant Prunus mongolica
SECHENBATER,MAN Liang,WANG Zhen xing,WU Tie jun,ZHANG Yu ping.Tissue culture and plantlet regeneration of endangered rare plant Prunus mongolica[J].Acta Botanica Boreali-Occidentalia Sinica,2002,22(6):1479-1481.
Authors:SECHENBATER  MAN Liang  WANG Zhen xing  WU Tie jun  ZHANG Yu ping
Abstract:Plantlets were regenerated from the endangered and rare plant Prunun mongolica Maxim via tissue eulture.The experiment results showed that the Prunus mongolica explants of shoot apex,shoot cutting section and leaf could be dedifferentiated to callus,and the callus could be redifferentiated to regeneration plantlet on the MS medium.The organs dedifferentiation and redifferentiation was determined by homone kinds and thier concentration in MS medium.The optimum medium of callus inducing was MS+6 BA 0.8 mg/L+NAA 0.1 mg/L,the optimum medium of inducing bud was MS+6 BA 0.8 mg/L.The optimum medium of rooting was MS+IBA 0.5 mg/L.
Keywords:Prunus mongolica  Maxim    endangered plant  tissue culture  plantlet regeneration
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