遮光灵武长枣果实糖积累和代谢相关酶活性特征

于灵武长枣盛花期对果实进行遮光处理,以自然照光为对照,通过测定果实生长指标、叶绿素含量、蔗糖代谢相关酶活性及其蔗糖代谢糖分含量等,研究果实光合作用在果实糖积累中的作用及对果实多糖和总糖含量积累的影响。结果表明:(1)遮光处理后,果实单粒重、单粒体积以及果实中叶绿素含量均降低。(2)遮光处理不同程度增加了果实中转化酶、蔗糖磷酸合成酶和蔗糖合成酶分解方向酶的活性,而降低了其蔗糖合成酶合成方向酶的活性。(3)遮光处理主要影响果实着色期和成熟期的糖含量,对果实发育初期糖含量影响较小;果实多糖的形成与果实所受光照状况具有一定的关系,而果实中总糖的积累与外界光照具有密切关系。可见,果实遮光处理影响了果实发育过程中蔗糖代谢相关酶的活性,从而影响果实糖分的代谢和积累。

Sugar Accumulation and Related Enzyme Activities in Shielding Light Fruit of Ziziphus jujuba Mill.cv.Lingwuchangzao

By contrast natural light fruit,the fruit of Ziziphus jujuba Mill.cv.lingwuchangzao were treated with shielding light at full flowering stage.The fruit growth index,chlorophyll content,enzyme activities of the sucrose metabolism and sugar content were measured to study the effects of photosynthesis on accumulation of fruit sugar and the content of polysaccharide and total sugar.The result showed that:(1)The weight and fruit volume of single fruit and content of chlorophyll reduced after shielding light treatment.(2)The activities of fruit invertase,sucrose phosphate synthase and the decomposition enzyme of sucrose synthase increased but the activities of the synthetic enzyme of sucrose synthase reduced with the different extent of shielding light treatment.(3)Shielding light treatment mainly affected sugar content in stages of color changing and ripe stage,but little effect on initial period of fruit development.In conclusion,the formation of polysaccharide in fruit had the certain relations to normal illumination,but the accumulation of total sugar in fruit had close relations to illumination.To sum up,shielding light treatment affected sugar metabolism and accumulation by influence enzyme activities of the sucrose metabolism in developmental fruit.