内蒙古地区白桦外生菌根形态类型及分子鉴定

以内蒙古不同地区白桦外生菌根为材料,采用形态解剖学方法和分子生物学手段对与白桦共生的外生菌根真菌多样性进行全面的调查,并经对所测序列与GenBank和Database比对。结果显示:在内蒙古地区与白桦共生的外生菌根真菌共13种,其中担子菌7种,子囊菌4种,分别来自于丝膜菌属、丝盖伞属、蜡壳耳属、毛革菌属、滑菇属和空团菌属、块菌属、地怀菌属。其中,菌根类型T₈和T₁₁未能提出其总DNA,根据其外生菌根形态类型并参照Agerer体视显微镜菌根图谱和Haug菌根图谱进行比较,分类鉴定为荷顿氏疣柄牛肝菌和白桦外生菌根真菌一种。结果表明,内蒙古地区白桦外生菌根真菌多样性相对较高,且与利用地上子实体鉴定的外生菌根真菌种类有一定的区别。

Morphological Type and Molecular Identification of Ectomycorrhizae on Betula platyphylla in Inner Mongolia Area

Uing the ectomycorrhizae on Betula platyphylla in Inner Mongolia different areas as experimental material.The primary study on the diversity of ectomycorrhizas associated with B.platyphylla in Inner Mongolia area was investigated using morphological and anatomical and molecular method,then identification results were obtained according that the sequences were BLASTed using the GenBank and UNITE database.The results indicated that there are 13 different ECM fungi on B.platyphylla in Inner Mongolia area.Among the 13 identified ectomycorrhizal fungi,4 species belong to Ascomycotina and the rest species belong to Basidiomycotina,they belong to Cortinarius,Inocybe,Sebacina,Piloderma,Hebeloma and Cenococcum,Tuber,Geopyxis,respectively and among 13 different types,T₈ and T₁₁ failed to extract the DNA.So according to the map of ectomycorrhizal fungi which were built by Agerer and Haug,the two types were identified as Leccinum holopus and Betularhiza luteopinnata.The results shown that the diversity of ECM on B.platyphylla in Inner Mongolia area is higher,and it is different with the result which was identified by fruiting body associated with B.platyphylla.