Radiation protection of DNA by ferulic acid under in vitro and in vivo conditions |
| |
Authors: | Dharmendra Kumar Maurya Veena Prakash Salvi Cherupally Krishnan Krishnan Nair |
| |
Institution: | (1) Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, India |
| |
Abstract: | The effect of ferulic acid was studied on γ-radiation-induced relaxation of plasmid pBR322 DNA and induction of DNA strand
breaks in peripheral blood leukocytes and bone marrow cells of mice exposed to whole body γ-radiation. Presence of 0.5 mM
ferulic acid significantly inhibited the disappearance of supercoiled (ccc) plasmid pBR322 with a dose modifying factor (DMF)
of 2.0. Intraperitoneal administration of different amounts (50, 75 and 100 mg/kg body weight) of ferulic acid 1 h prior to
4 Gy γ-radiation exposure showed dose-dependent decrease in the yield of DNA strands breaks in murine peripheral blood leukocytes
and bone marrow cells as evidenced from comet assay. The dose-dependent protection was more pronounced in bone marrow cells
than in the blood leukocytes. It was observed that there was a time-dependent disappearance of radiation induced strand breaks
in blood leukocytes (as evidenced from comet parameters) following whole body radiation exposure commensuration with DNA repair.
Administration of 50 mg/kg body weight of ferulic acid after whole body irradiation of mice resulted disappearance of DNA
strand breaks at a faster rate compared to irradiated controls, suggesting enhanced DNA repair in ferulic acid treated animals.
(Mol Cell Biochem xxx: 209–217, 2005) |
| |
Keywords: | comet assay DNA repair ferulic acid radioprotection |
本文献已被 PubMed SpringerLink 等数据库收录! |