Stimulation and inhibition of cardiac myocyte proliferation in vitro |
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Authors: | Elissavet Kardami |
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Institution: | (1) St. Boniface General Hospital Research Centre, Division of Cardiovascular Sciences, University of Manitoba, 351 Tache Avenue, R2H 2A6 Winnipeg, Manitoba, Canada |
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Abstract: | Summary We have examined the effect of crude cardiac tissue extracts as well as that of several growth factors and triiodothyronin (T3) on DNA synthesis of cardiac myocytes in culture. Extracts from embryonic and adult cardiac tissue stimulated DNA synthesis of myocytes. Atrial myocytes exhibited overall higher degree of stimulation than their ventricular counterparts and extracts from adult atrial tissue had the highest apparent mitogenic activity for atrial myocytes. We have shown that adult heart contains basic fibroblast growth factor (bFGF), especially in the atria 1]. Transforming growth factor (TGF) and insulin-like growth factors (IGFs) are also accumulated in cardiac tissues 2, 3]. We found that bFGF and the IGFs stimulate myocyte cell proliferation and DNA synthesis. These factors also stimulate cardiac non-muscle proliferation, especially in the presence of serum. TGF inhibited proliferation and DNA synthesis and cancelled the effect of bFGF or IGFs on the myocytes. T3 also diminished the bFGF-induced mitogenic stimulation of cardiomyocytes. Our data suggest that these factors may be involved in the regulation of cardiomyocyte proliferation in vivo.Abbreviations bFGF
basic Fibroblast Growth Factor
- BSA
Bovine Serum Albumin
- DM
Defined Medium
- Fes
Fetal calf serum
- FITC
Fluorescein
- IGF
Insulin-like Growth Factor
- IgG
Immunoglobulin
- LI
Labeling Index
- PBS
Phosphate Buffered Saline
- T3
Triiodothyronine
- TGF
Transforming Growth Factor |
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Keywords: | cardiocytes cell proliferation growth factors DNA synthesis |
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