Effects of repeated desflurane and sevoflurane anesthesia on enzymatic free radical scavanger system |
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Authors: | Bayazit Dikmen Yusuf Unal H Kutluk Pampal Nilhan Nurlu Omer Kurtipek Orhan Canbolat Candan Özoğul Mustafa Kavutcu |
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Institution: | (1) Ankara Numune Hospital, Department of Anesthesiology & Reanimation, Gazi University Medical Faculty, 06510, Beşevler, Ankara, Turkiye;(2) Department of Anesthesiology & Reanimation, 06510, Beşevler, Ankara, Turkiye;(3) Medical Biochemistry Department, 06510, Beşevler, Ankara, Turkiye;(4) Department of Histology and Embriology, 06510, Beşevler, Ankara, /Turkiye;(5) Medical Biochemistry Department, Gazi University Medical Faculty, 06510 Beşevler/Ankara, Turkiye |
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Abstract: | Background: To investigate the possible effects of repeated sevoflurane and desflurane anesthesia on hepatocellular system by evaluating
the free radical metabolism, hepatocellular enzymes and histopatholgical changes in rats. Methods: Four groups of animals were studied. Sevoflurane 2% (v/v) and desflurane 6% (v/v) in air/O2 were administered to animals in group II (n = 9) and III (n = 9) respectively. 100% (v/v) O2 was administered in group IV (n = 9). Administration was done for 60 minutes over 3 days. Nine animals were allocated to control group (group I), superoxide
dismutase (SOD), catalase (CAT), glutathion peroxidase (GSH-Px), glutathione-s-transferase (GST) and thiobarbituric acid reactive
substances (TBARS) were studied. Also electron microscopy was performed. Results: Catalase, SOD, GSH-Px, GST activities and TBARS levels were significantly higher in groups II and III than in group I (p < 0.05). All parameters were significantly higher in groups II versus group IV (p < 0.05). On the other hand, SOD, GSH-Px and GST activities were significantly elevated in group III than IV, but CAT activity
and TBARS levels were not significantly. Catalase, SOD, GSH-Px, GST but not TBARS levels were significantly higher in groups
II and III than in group IV (p < 0.05). TBARS levels were higher in group III than in group IV, but this elevation was not statistically significant. CAT,
SOD and GSH-Px activities were significantly higher in groups II than in group III (p < 0.05). Conclusion: Although electron microscopy findings were similar for group II and III, we can conclude that sevoflurane might cause more
cellular damage than desflurane by causing higher activation of free radical metabolising enzymes. |
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Keywords: | free radicals sevoflurane desflurane hepatotoxicity glutathione-S-transferase antioxidant enzymes |
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