Vanadium salts stimulate mitogen-activated protein (MAP) kinases and ribosomal S6 kinases |
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Authors: | Sanjay K Pandey Jean-Louis Chiasson Ashok K Srivastava |
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Institution: | (1) Research Group On Diabetes and Metabolic Regulation, Centre de Recherche/Hotel-Dieu de Montreal Hospital, 3850 Saint-Urbain Street, H2W IT8 Montreal, Quebec, Canada;(2) Department of Medicine, University of Montreal, 3850 Saint-Urbain Street, H2W IT8 Montreal, Quebec, Canada |
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Abstract: | Effect of several vanadium salts, sodium orthovanadate, vanadyl sulfate and sodium metavanadate on protein tyrosine phosphorylation and serine/threonine kinases in chinese hamster ovary (CHO) cells overexpressing a normal human insulin receptor was examined. All the compounds stimulated protein tyrosine phosphorylation of two major proteins with molecular masses of 42 kDa (p42) and 44 kDa (p44). The phosphorylation of p42 and p44 was associated with an activation of mitogen activated protein (MAP) kinase as well as increased protein tyrosine phosphorylation of p42mapk and p44mapk. Vanadinm salts also activated the 90 kDa ribosomal s6 kinase (p90rsk) and 70 kDa ribosomal s6 kinase (p70s6k). Among the three vanadium salts tested, vanadyl sulfate appeared to be slightly more potent than others in stimulating MAP kinases and p70s6k activity. It is suggested that vanadium-induced activation of MAP kinases and ribosomal s6 kinases may be one of the mechanisms by which insulin like effects of this trace element are mediated.Abbreviations eIF-4
eukaryotic protein synthesis initiation factor-4
- GRB-2
growth factor receptor bound protein-2
- GSK-3
Glycogen Synthase Kinase-3
- IRS-1
insulin receptor substrate-1
- ISPK
insulin stimulated protein kinase
- MAPK
mitogen activated protein kinase, also known as
- ERK
extracellular signal regulated kinase
- MAPKK
mitogen activated protein kinase kinase, also known as-MEK, MAPK or ERK kinase
- PHAS-1
phosphorylated heat and acid stable protein regulated by insulin
- PI3K
phosphatidyl inositol 3-kinase
- PP1-G
protein phosphatase-glycogen bound form
- PTK
protein tyrosine kinase
- PTPase
protein tyrosine phosphatase
- rsk
ribosomal s6 kinases
- shc
src homology domain containing protein
- SOS
son of sevenless |
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Keywords: | vanadium salts MAP kinase ribosomal s6 kinases (p90rsk and p70s6k) insulinomimesis Protein tyrosine phosphates |
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