Reduced troponin I phosphorylation and increased Ca2+-dependent ATP-consumption in triton X-skinned fiber preparations from Gαq overexpressor mice |
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Authors: | C Pott L Willkomm S Grafweg B B?lck G W Dorn nd R H G Schwinger K Brixius |
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Institution: | (1) Division of Biological Sciences, Chonbuk National University, Jeonju, 561-756, Korea;(2) Institute of Oral Biosciences, Chonbuk National University, Jeonju, 561-756, Korea;(3) Research Center of Bioactive Materials, Chonbuk National University, Jeonju, 561-756, Korea;(4) Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Korea University, Seoul, 152-050, Korea;(5) National Livestock Research Institute, RDA, Suweon, 441-706, Korea;(6) Department of Animal Resources and Biotechnology, Chonbuk National University, Jeonju, 561-756, Korea |
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Abstract: | Mechanical stress leads to satellite cell activation, which is an important event in the development, growth, and remodeling
of postnatal skeletal muscle. Although there is a considerable knowledge on the events involved in skeletal muscle regeneration
and development, the precise role of mechanical stress on activation of satellite cells remains unclear. Previously, satellite
cells were isolated from adult bovine muscle and it was shown that the cells are multipotent, i.e., capable of proliferating
and to differentiating into both myoblasts and adipocytes. This study investigated the cellular mechanisms by which cyclic
mechanical stretching modulates the proliferation and differentiation of adult bovine satellite cells. The application of
cyclic stretch induced the proliferation of satellite cells and inhibited their differentiation into myotubes. This response
is believed to be closely related to the stretch-mediated changes in the expression of myogenic and cell cycle regulatory
factors. Cyclic stretching increased the level of extracellular signal-regulated kinase (ERK) phosphorylation, whereas a specific
ERK inhibitor (PD98058) blocked the stretch-mediated inhibition of myogenesis in a dose-dependent manner. Overall, this study
demonstrates for the first time that cyclic mechanical stretch induces the proliferation of bovine satellite cells and suppresses
their myogenic differentiation through the activation of ERK. |
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Keywords: | Adult bovine satellite cells Cyclic mechanical strain Myogenesis Muscle regulatory factors Extracellular signal-regulated kinase |
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