Thyroid hormone effects on the State IV proton motive force in rat liver mitochondria |
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Authors: | Mark A Horrum Richard B Tobin Robert E Ecklund |
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Institution: | (1) Department of Internal Medicine, College of Medicine, University of Nebraska Medical Center and Research Service, USA;(2) Department of Veterans Affairs, Medical Center, 68105 Omaha, Nebraska, USA;(3) D.V.A.M.C. Research Service R-217, 4101 Woolworth Avenue, 68105 Nebraska, Omaha, USA |
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Abstract: | In order to further investigate the mechanisms regulating the control of mitochondrial respiration by thyroid hormone, the proton motive force was measured during State IV respiration in liver mitochondria isolated from euthyroid, hyperthyroid, hypothyroid and T3-treated hypothyroid rats. The proton motive force was significantly higher in the hyperthyroid group due to an increased pH. The proton motive force of hypothyroid mitochondria was lower than controls due to a decreased membrane potential. The proton motive force for the T3-treated hypothyroid group did not differ from the euthyroid group due to negating changes in the pH gradient and the membrane potential. The intramitochondrial volume was decreased in the hyperthyroid group and unchanged in the other groups. The results indicate that the thyroid status alters the proton motive force in State IV through individual changes in the pH and membrane potential components of the force. The component that changes in hyperthyroid mitochondria is different from that changing in hypothyroid mitochondria. |
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Keywords: | mitochondria thyroid hormone proton motive force State IV rat liver |
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