Upregulation of AP1 by tertiary butyl hydroperoxide induced oxidative stress and subsequent effect on spermatogenesis in mice testis |
| |
Authors: | Parminder Kaur Gagandeep kaur Mohinder P Bansal |
| |
Institution: | (1) Department of Biophysics, Panjab University, Chandigarh, 160014, India |
| |
Abstract: | Oxidative stress is detrimental to sperm function and a significant factor in the etiology of male infertility. Present study
evaluates the effect of ter butyl hydroperoxide (TBHP)-induced oxidative stress on the spermatogenic process and cell number
in the seminiferous tubules. Intraperitoneal injection of TBHP (84 μmol TBHP/100 g body weight) for 2 weeks to male Balb/c
mice resulted in enhanced lipid peroxidation (P < 0.0001) decrease in reduced glutathione (P < 0.0001) and increase in the oxidized glutathione levels (P = 0.007) in the testis. Status of spermatogenesis after the treatment was assessed by the quantitative methods of germ cell
evaluation in the seminiferous tubules. A significant decrease in the number of young spermatids (P = 0.0003) and pachytene cells (P = 0.022) was observed. A marked reduction was also seen in the mature spermatid number (P < 0.0001). An increase in testicular mRNA levels of redox-regulated cjun (P = 0.008) and cfos (P = 0.0006) subunits of activator protein 1 (AP1) was observed after TBHP treatment. Evaluation of AP1 regulated antioxidant
enzymes in the testis revealed an increase in γ-glutamyl cysteine synthetase (GCS) mRNA expression (P = 0.001). These results suggest a potential role of AP1 in oxidative stress-mediated meiotic and post meiotic changes in
the spermatogenic process and regulation of cell number in male reproductive system. |
| |
Keywords: | cfos cjun Oxidative stress TBHP Testis |
本文献已被 PubMed SpringerLink 等数据库收录! |