| 根癌农杆菌转化紫草的研究 |
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| 引用本文： | 祁建军,宋经元,雷和田,张荫磷.根癌农杆菌转化紫草的研究[J].云南植物研究,2002,24(5):656-658. |
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| 作者姓名： | 祁建军 宋经元 雷和田 张荫磷 |
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| 作者单位： | 中国医学科学院药用植物研究所,中国协和医科大学,北京,100094 |
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| 摘 要： | 紫草 (ＬｉｔｈｏｓｐｅｒｍｕｍｅｒｙｔｈｒｏｒｈｉｚｏｎＳｉｅｂ .ｅｔＺｕｃｃ)是传统中药。其根部含有萘醌类化合物—紫草素及其衍生物 ,具有显著的抗菌、抗炎、抗癌以及促进伤口愈合等生理活性。紫草素同时也是一种名贵化妆品染料。科学家对紫草的研究兴趣是基于其资源的缺乏及紫草植物本身所具有的一些特点 ;如 :紫草素及其衍生物的颜色特性可凭借肉眼观察 ,紫草素及其衍生物只在紫草的根部积累 ,紫草素合成的次生代谢途径受多种酶和外界条件 (光照 ,营养等 )的调节等。紫草细胞培养 (Ｆｕｊｉｔａ等 ,1983;叶和春等 ,1991)可以产…
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| 关 键 词： | 根癌农杆菌 转化 紫草 冠瘿组织培养 红色株系 |
| 文章编号： | 0253-2700(2002)05-0656-03 |
| 修稿时间： | 2002年3月11日 |
Study on the Genetic Transformation of Lithospermum erythrorhizon by Agrobacterium tumefaciens |
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| QI Jian Jun,SONG Jing Yuan,LEI He Tian,ZHANG Yin Ling.Study on the Genetic Transformation of Lithospermum erythrorhizon by Agrobacterium tumefaciens[J].Acta Botanica Yunnanica,2002,24(5):656-658. |
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| Authors: | QI Jian Jun SONG Jing Yuan LEI He Tian ZHANG Yin Ling |
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| Abstract: | Crown gall tissue culture lines of Lithospermum erythrorhizon were established by transformation of sterile seedlings with Agrobacterium tumefaciens C58. Nopaline was inspected in the crown gall and showed that the transformation was successfull. L6 line, can secret red naphthoquinone pigments that are shikonin derivatives on phytohormone free 6,7-V medium, was selected from other lines on different basic phytohormone free media(MS, 6,7-V, WP, B5).The content of shikonin in the crown gall was 1 18%(dry tissue).The study showed that crown gall tissue culture of L.erythrorhizon was a new method to produce shikonin. |
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| Keywords: | Lithospermum erythrorhizon Agrobacterium tumefaciens Crown Gall Tissue Culture Shikonin |
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