| 从天然提取物或分离部位中以烯醇式丙酮酸转移酶为靶点的抗细菌活性筛选 |
| |
| 引用本文： | 姜立花,谭宁华,杨亚滨,汪俊松,付祥,Lars JOHANNSEN,Hartwig MUELLER,Thomas HENKEL.从天然提取物或分离部位中以烯醇式丙酮酸转移酶为靶点的抗细菌活性筛选[J].云南植物研究,2003,25(1):90-94. |
| |
| 作者姓名： | 姜立花 谭宁华 杨亚滨 汪俊松 付祥 Lars JOHANNSEN Hartwig MUELLER Thomas HENKEL |
| |
| 作者单位： | 1. 中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南,昆明,650204
2. Bayer AG, PH-R-EU, D-42096 Wuppertal,德国 |
| |
| 基金项目： | ProjectsupportedbytheAgreementbetweenKunmingInstituteofBotany(ChineseAcademyofSciences，Chi na)andBayerAG (Germany) (BK -95 01)，theMinistryofScience&TechnologyofChina (973 :G19980 1113，863 :2 0 0 1AA2 3 40 11-0 2ChineseAcademyofSciences(KSCX1-0 9-0 3 -1) |
| |
| 摘 要： | 目的是以烯醇式丙酮酸转移酶(EPT)为靶点筛选其抑制剂，以期寻找抗细菌活性样品。实验是在96％孔酶标板上对来源于169个科、560个属、916种动植物2490个提取物或分离部位样品在EPT模型上进行了批量筛选。结果表明在96.15μg/ml浓度下发现了来源于80个科、169个届、218个种的309个样品有活性，其中14个样品的IC50小于10．00μg/ml，40个样品的IC50在10．01-30．00μg/ml范围，83个样品的IC50在30．01—50．00μg/ml范围，172个样品的IC50在50．01—96.15μg/ml范围。通过以上工作我们认为以烯醇式丙酮酸转移酶为分子靶点的体外筛选方法稳定、方便、快速、微量、有效，特别适用于天然产物的抗细菌活性筛选。
|
| 关 键 词： | 烯醇式丙酮酸转移酶 抑制剂 抗细菌活性 天然产物 天然提取物 分离部位 体外筛选 |
Searching for Antibacterial Activities of Extracts and Fractions Derived from Natural Sources Targeting Enoylpyruvate Transferase |
| |
| Lars JOHANNSEN,Hartwig MUELLER,Thomas HENKEL.Searching for Antibacterial Activities of Extracts and Fractions Derived from Natural Sources Targeting Enoylpyruvate Transferase[J].Acta Botanica Yunnanica,2003,25(1):90-94. |
| |
| Authors: | Lars JOHANNSEN Hartwig MUELLER Thomas HENKEL |
| |
| Abstract: | To discover inhibitors of enoylpyruvate transferase with antibacterial activity a batch of 2490 extract or fraction samples prepared from plants and animals belonging to 169 families, 560 genera and 916 species were tested on enoylpyruvate transferase bioassay in 96-well microtiterplates. Finally 309 samples, which belong to 80 families, 169 genera and 218 species, showed inhibitory activity at 96.15 μg/ml, in which 14 samples showed IC50 at=<10.00 μg/ml, 40 samples showed IC50 at 10.01-30.00 μg/ml, 83 samples showed IC50 at 30.01-50.00 μg/ml and 172 samples showed IC50 at 50.01-96.15 μg/ml.It is indicated that this in-vitro bioassay is convenient, stable, rapid, sensitive and effective in searching for antibacterial activity samples from natural sources. |
| |
| Keywords: | Antibacterial activity screening Enoylpyruvate transferase Natural products Extracts Fractions |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
