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库拉索芦荟的多倍体诱导及其变异初报
引用本文:王俐,郑思乡,李枝林,顾志建.库拉索芦荟的多倍体诱导及其变异初报[J].云南植物研究,2001,23(4):493-496.
作者姓名:王俐  郑思乡  李枝林  顾志建
作者单位:1. 云南农业大学花卉研究所,云南,昆明,650201
2. 中国科学院昆明植物研究所,云南,昆明,650204
摘    要:在组织培养条件下,对库拉索芦荟(Aloe vera L.)用秋水仙素进行染色体加倍的诱导处理,结果表明:用含0.06%秋水仙素处理12h后诱变频率可达50%,其效果最佳。经秋水仙素诱导的加倍群体与正常二倍体植株比较,植株的大多数叶片变厚,叶色变深,叶片变大,气孔增大而单位叶面积气孔数减少。对变异材料进行细胞学研究所发现,体细胞中期染色体为2n=4x=28,为4倍体。未加倍前的二倍体为2n=2x=14。检测中也发现有少数植株有2n=14和2n=28两种细胞型的情况。

关 键 词:库拉索芦荟  多倍体  诱导  形态学  细胞学
文章编号:0253-2700(2001)04-0493-04
修稿时间:2000年10月9日

A Preliminary Study on the Polyloid Induction and Variation of Aloe vera
WANG Li ,ZHENG Si-Xiang ,LI Zhi-Lin ,GU Zhi-Jian.A Preliminary Study on the Polyloid Induction and Variation of Aloe vera[J].Acta Botanica Yunnanica,2001,23(4):493-496.
Authors:WANG Li  ZHENG Si-Xiang  LI Zhi-Lin  GU Zhi-Jian
Institution:WANG Li 1,ZHENG Si-Xiang 1,LI Zhi-Lin 1,GU Zhi-Jian 2*
Abstract:Tube seedlings of Aloe vera L. were treated with colchicine to from polyploid plants. The results show that the best induction rate could reach 50% after treatment with 0.06% colchcine in 12 hours. By comparing the polyploid plants with normal diploid ones, leaves of polyploid plants were thicker, larger, darker in color, and with larger stomata and less in number. Cytological studies show that the chromosome number of the variant is 28, while the normal diploid plant is 14. It was found that a few induced plants were chimera with diploid cells (2n=4x=14) and tetraploid cells (2n=4x=28) simultaneously.
Keywords:Aloe vera  Polyploid  Induction  Morphology  Cytology
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