慈竹构件和分株水平总黄酮含量的变化

黄酮类化合物是植物体内重要的次生代谢物质之一。本文采用AlCl3 比色法测定了慈竹构件和分株水平总黄酮含量的变化 ,以探讨其生理生态适应性。结果表明 ,在地上 3个构件中 ,叶的总黄酮含量显著大于枝和秆 ;1龄分株叶的总黄酮含量显著大于其它 4个龄级 ,而枝和秆的含量在 5个年龄间无显著差异 ;分株上层叶和枝条的总黄酮含量显著多于中下两层的相应构件。叶龄对慈竹叶总黄酮含量有显著的影响 ,1龄、 7龄和 9龄叶片的总黄酮含量明显高于其它 6个龄级 ,但是叶龄和总黄酮含量之间无显著线性相关。同时 ,叶总黄酮含量对季节的变化敏感 ,2、 4和 12月含量最高 ,5月和 8月含量最低。在分株水平 ,1龄分株的总黄酮含量显著大于其它 4个龄级。这些结果说明慈竹的总黄酮含量对环境变化具有可塑性 ,分株水平与叶构件水平总黄酮含量对分株年龄的反应格恐相同。

Changes of Total Flavonoids Content at the Module and Ramet Levels in Neosinocalamus affinis

Flavonoid is one of the most important secondary metabolites in plants.In order to understand physiological and ecological adaptation mechanism of clonal plant Neosinocalamus affinis,the effects of biological factors (module,ramet age,ramet position and leaf age) and non-biological factor (season change) on total flavonoids content were analyzed by the method of AlCl 3 colorimetry. The results showed that total flavonoids content decreased in the order of leaf >branch>culm at the module level.Total flavonoids content of leaves in 1-year-old ramets was much higher than the other of four age ramets,but that of shoots and culms was not significantly different among different age ramets.Total flavonoids content of leaf and branch at upper part of ramets had an advantage over those of middle and lower part.It demonstrated that more flavonoids were likely to be a protective strategy in response to strong light. Leaves aged 1,7 and 9 contained more flavonoids than those aged 2-6 and 8.But there was not negative correlation between leaf age and total flavonoids content.Leaf adhered to a monthly change,in which total content peaked in February,April and December while the lowest point appeared during May to August. At the ramet level,total flavonoids content of 1-year-old ramets was significantly higher than that of the other four age ramets. The above-mentioned results suggested that:(1)total flavonoids content of Neosinocalamus affinis was affected by environmental conditions such as light intensity,season change and so on.(2) total flavonoids content has no hierarchical response to ramet age at the leaf module and ramet levels in Neosinocalamus affinis.Finally,We discussed the mechanism of total flavonoids content in response to environmental changes.