植物种质资源超低温保存概述

简要回顾了植物种质资源超低温保存的历史，说明了超低温保存植物材料的多样性，阐述了超低温耐性的生物学基础及超低温伤害产生的原因和类型，介绍了各种常用超低温保存方法的技术要点，并对生产顽拗性种子的植物种质资源的超低温保存作了专门的论述，分析了生产顽拗性种子的植物种质资源超低温保存的潜力、现状和困难，指出顽拗性种子的超低温保存是植物种质资源超低温保存的重点和难点，而真正实现用超低温保存技术贮藏顽拗性植物种质资源还有很长的路要走。

An Introduction to Cryopreservation of Plant Germplasm

This paper recited the history of plant germplasm cryopreservation, exhibited the diversity of cryopreservated plant materials, analysed biological foundation of plant cryotolerance, causes and types of cryo-injury, described the key techniques of frequently-used cryopreservation methods, and paid special attention to cryopreservation of plant germplasm of species producing recalcitrant seeds. Based on analysis of potential, status and difficulties, the author thought that regard to cryopreservation of plan...