ABA对黑黄檀种子萌发的抑制作用以及其他植物激素对ABA的拮抗作用

以云南特有濒危树种黑黄檀(Dalbergia fusca)的种子为材料,研究了脱落酸(ABA)对种子萌发的抑制作用,以及种子萌发过程中吲哚乙酸(IAA)、赤霉酸(GA_3)、6-苄基腺嘌呤(6-BA)和乙烯利对ABA的拮抗作用.黑黄檀种子萌发的适宜温度为30℃.交替光照(14 h光照和10 h黑暗)以及黑暗对种子萌发没有明显的影响.0.001～0.1 mmol/L ABA不影响种子的萌发率,但降低种子的萌发进程;1 mmol/L和2.5mmol/L ABA显著地抑制种子的萌发率和萌发进程.种子的萌发率不被0.0001～1 mmol/L IAA和GA3、0.0001～0.1 mmol/L 6-BA、以及0.001～10 mmol/L 乙烯利(乙烯供体)的影响,但被1 mmol/L 6-BA抑制.1mmol/L ABA对种子萌发的抑制作用能被0.01～1 mmol/L IAA、0.01～1 mmol/L GA3、0.001～0.1 mmol/L 6-BA和0.1～10 mmol/L乙烯利所拮抗,而且这种拈扰作用与植物激素的类型和浓度有关.0.01 mmol/L 6-BA和0.1 mmol/L乙烯利对l mmol/L ABA抑制作用的拈抗不能被添加0.001 mmol/L IAA或者O.001 mmol/L GA3加成.但0.1 mmol/L 乙烯利对1 mmol/L ABA抑制作用的拮抗能够被添加O.01 mmol/L 6-BA或者0.1 mmol/L 6-BA加成,导致更高的萌发率和幼苗生长.

Inhibitory Effect of ABA on Seed Germination of Dalbergia fusca (Leguminosae)and Antagonism of Other Phytohormones to ABA

Seeds of Dalbergia fusca, an endangered tree species endemic to Yunnan province of China were used to study the inhibitory effects of abscisic acid (ABA) and the antagonism of IAA, GA3, 6-BA and ethephon to ABA in the seed germination. The optimum temperature for the seed germination was about 30℃. There was no different effects of alternating photoperiod ( 14 h hght and 10 h dark) and darkness on the seed germination. After treated by 0.01-0.1 mol/L ABA,the seed germination percentage was not affected, but the time course of germination was decreased, while those levels were dramatically inhibited by 1 mmol/L and 2.5 mmol/L ABA. The seed germination percentage was not affected by 0.0001-1 mmol/L indole-3-aeetic acid (IAA), gibberellie acid (GA3), 0.0001-0.1 mmol/L 6-benzyladenine (6-BA), and 0.001-10 mmol/L ethephon (the ethylene donor), but was inhibited by 1 mmol/L 6-BA. The inhibition effect of I mmol/L ABA on seed germination was antagonized by 0.01-1 mmol/L IAA, 0.01-1 mmol/L GA3,0.001-0.1 mmol/L 6-BA, and 0.1 -10 mmol/L ethephon, which were phytohormone type- and concentration-dependent. The antagonistic actions of 0.01 mmol/L 6-BA and 0.1 mmol/L ethephon to 1 mmol/L ABA inhibition could not be increased by addition of 0.001 mmnol/L IAA or 0.001 mmol/L GA3. The antagonistic action of 0.1 mmol/L ethephon to 1 mmol/L ABA inhibition, however, could be increased by addition of 0.01 mmol/L 6-BA or 0.1 mmol/L 6-BA, which resulting in higher germination percentage and enhancing seedling growth.