| 黑节草未成熟种子的形态发育及其在离体培养时的表现 |
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| 引用本文： | 叶秀粦,程式君,王伏雄,钱南芬.黑节草未成熟种子的形态发育及其在离体培养时的表现[J].云南植物研究,1988(3). |
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| 作者姓名： | 叶秀粦 程式君 王伏雄 钱南芬 |
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| 作者单位： | 中国科学院华南植物研究所 广州
(叶秀粦，程式君)，中国科学院植物研究所 北京
(王伏雄)，中国科学院植物研究所 北京(钱南芬) |
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| 摘 要： | 黑节草(Dendrobium candidum Wall ex Lind.)2—6个月种龄的胚均处于球形胚阶段,不同种龄的胚在体积大小、胚细胞数目、胚细胞内的淀粉粒含量和超微结构上有差异。在离体培养条件下黑节草种子萌发率可达95％,种子萌发后形成原球茎,原球茎可以直接发育形成幼苗,又可以由原球茎产生大量愈伤组织,由愈伤组织再分化发育成幼苗。种子萌发过程中,胚顶端分生组织细胞的淀粉逐渐消耗,淀粉的变化与分生组织和子叶的形成有明显的相关性。
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| 关 键 词： | 黑节草 种子离体培养 胚胎发生 |
MORPHOLOGY OF IMMATURE SEEDS AND DEVELOPMENT IN VITRO OF DENDROBIUM CANDIDUM |
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| Yc Xiulin,Cheng Shijun.MORPHOLOGY OF IMMATURE SEEDS AND DEVELOPMENT IN VITRO OF DENDROBIUM CANDIDUM[J].Acta Botanica Yunnanica,1988(3). |
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| Authors: | Yc Xiulin Cheng Shijun |
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| Abstract: | The embryo of Dendrobium candidum Wall ex Lindl. in different stages is globular. But embryos arc different in shape, cell numbers, starch grains and ultrastrucfures from fruit during two to six months after pollination. Seed germination rate is 95% in vitro, Protocorms are formed from embryos. The protocorm can directly form seedling or callus. The callus may differentiate inko seedling again. During seed germination the starch is reduced gradually in the cells at the top of the embryo. The reduction of starch may be closely related to the mcristem and the formation of the cotyledon. |
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| Keywords: | Dendrobium candidum Seed germination in vitro Embryo-genesis |
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