应用过氧化物酶同工酶鉴定山茶属植物杂种F1代的研究

对4个杂交组合的12个杂种F1代植株与亲本进行了形态学比较和过氧化物酶同工酶分析,结果表明以金花茶为父本,云南山茶或云南野山茶为母本杂交产生的11个杂种的幼枝、叶的特征均与母本相似,H-86-1-1的花与母本一致,H-87-2-2的花兼有父母本的特征;H-78-1-1的幼枝、叶的特征与父本相似,其花也兼有父母本的特征。有9株的酶谱为“互补型酶带”,且都出现了杂种带,为真正的杂种;其余2株的酶谱与母本一致,与其形态特征表现出一定的相关性,可能为非真正的杂种。同一个杂交组合产生的杂种,酶谱却有差异,金花茶最为特征的一条带(Rf=0.813)在其所有11个杂种F1代植株中都未表达。

APPLICATION OF PEROXIDASE ISOZYMES TO IDENTIFICATION F1 HYBRIDS OF CAMELLIA

In this paper 12 F1 hybrids of four combinations were compared with their parents in morphology and peroxidase isozymes, the results indicated that: (1) Certain morphological characters may be used for initial identification of Fl hybrids. (2) Nine of all hybrids display both parental bands except one peculiar band of C. chrysantha, and possess "hybrid" - bands not observed in either parent; zymograms of the two other plants are the same as female parents, possibly not true hybrids. (3) The Fl hybrids produced from C. chrysantha, C. reticulata or C. pitardii var. yunnanica express various zymograms. Based on the above, we propose that it is a much effective method to identify F1 hybrids by using peroxidase isozyme variations.