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| 姜花属SRAP分子标记连锁图谱构建 | |
| 引用本文： | 高丽霞,刘念,黄邦海.姜花属SRAP分子标记连锁图谱构建[J].云南植物研究,2009,31(4):317-325. |
| 作者姓名： | 高丽霞 刘念 黄邦海 |
| 作者单位： | 1. 河池学院化学与生命科学系,广西,宜州,546300 2. 仲恺农业工程学院,广东,广州,510225 3. 广州市农业技术推广中心,广东,广州,510520 |
| 基金项目： | 广西高校重点建设实验室建设资助项目 |
| 摘 要： | 采用拟测交作图策略，利用白姜花×圆瓣姜花的F1群体87个单株，分别构建了父母本的基于SRAP标记的连锁图谱。通过筛选，414对引物中，92对引物可以检测到拟测交位点。在检测到的398个拟测交位点中，237个来自于父本圆瓣姜花，161个来自于母本。经过卡方（x^2）测验及连锁分析，父本中，203个标记进入23个连锁群，覆盖了1386．8cm；母本中，139个标记进入18个连锁群，覆盖了917．1cm。 |
| 关 键 词： | F1群体 连锁图谱 拟测交作图 |
Linkage Maps of the Genus Hedychium (Zingiberaceae) Based on SRAP |
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| GAO Li-Xia,LIU Nian,HUANG Bang-Hai.Linkage Maps of the Genus Hedychium (Zingiberaceae) Based on SRAP[J].Acta Botanica Yunnanica,2009,31(4):317-325. | |
| Authors: | GAO Li-Xia LIU Nian HUANG Bang-Hai |
| Institution: | GAO Li-Xia, LIU Nian , HUANG Bang-Hai (1. Department of Chemistry and Life Science, Hechi University, Yizhou 546300, China; 2. Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; 3. Guangzhou Municipal Center for the Promotion of Agricuhural Technologies, Guangzhou 510520, China) |
| Abstract: | In this study, using a pseudo-cross strategy, we constructed two maps of Hedyhium, Hedychium coronarium and Hedychium forrestii based on SRAP markers. The mapping population consisted of 87 progenies, from a F1 population. A total of 414 primer pairs were screened and 92 pairs were considered. Among 398 loci, 237 loci were from the paternal parent and 161 were from the maternal parent. After x^2 test and linkage analysis, two maps were constructed as following： The paternal parent contained 203 loci and spanned 1 386.8 cm, which spread over 23 hnkage groups. The maternal parent contained 139 loci and spanned 917.1 cm. These loci were distributed in 18 linkage groups. |
| Keywords: | F1 population Llinkage map Pseudo-cross strategy |
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