不同抗冷性水稻中编码甘油-3-磷酸转酰酶的部分cDNA的序列比较研究

采用ＲＴ－ＰＣＲ技术,以根据国外报道的几种双子叶植物的甘油－３－磷酸转酰酶的桎保守的氨基酸而设计的简并引物作为扩增引物,从不同抗冷性水稻品种中均扩增到一段约３１５ｂｐ的ｃＤＮＡ片段。测序结果表明它们都是编码ＧＰＡＴ的部分ｃＤＮＡ含有３１５个核苷酸,编码１０５个氨基酸。比产它们之间的核苷酸及推导氨基酸序列,发现有一定差异,且抗冷性相差越大的品种间差异越大。抗冷性的差异可能与脯氨枝的替换有关。

Sequence Comparison of Part cDNAs Coding for Glycerol-3- Phosphate Acyltransfera from Rice Strains with Different Chilling-Resistance

Using RT-PCR technique, we obtained a fragment of cDNA about 315bp from rice strains with different chilling-resistance. In amplification reactions, the used primers are degenerated primers, which were designed according to the conserved amino acid sequences of glycerol-3-phosphate acyltransferase from several kinds of dicotyls. Sequences obtained show that the cDNAs fragments all have the parts of cDNAs coding for Glycerol-3-phosphate acyltransferase, contain 315 nucleotides and encode 105 amino acids. While the cDNAs sequences and deduced amino acids sequences from the rice strains with different chilling-resistance were different from each other. The bigger difference of chilling-resistance between strains is, the bigger differences between their cDNAs sequences and the deduced amino acids sequences are. The difference of chilling-resistance is probably related to the subtitution of proline.