| 双向电泳分析鸢尾绿白嵌合叶片的蛋白质 |
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| 引用本文： | 胡金勇,曾英,桑玉英.双向电泳分析鸢尾绿白嵌合叶片的蛋白质[J].云南植物研究,2002,24(3):387-391. |
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| 作者姓名： | 胡金勇 曾英 桑玉英 |
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| 作者单位： | 中国科学院昆明植物研究所植物生物技术室,云南,昆明,650204 |
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| 基金项目： | 云南省自然科学基金资助项目 (1999C0 0 77M) |
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| 摘 要： | 利用双向聚丙烯酰胺凝胶电泳对鸢尾（Iris japonica)绿白嵌合叶片的蛋白质进行分离，并初步鉴定了蛋白质的相对分子量和等电点。每个电泳图谱共检测到400余个蛋白点，其中至少13个蛋白的表达变化明显；结果表明，嵌合叶片的绿色与白色叶组织具有明显不同的蛋白质双向电泳图谱。与数据库中拟南芥双向电泳图谱相比较，发现Rubisco大亚基，标记为W和T蛋白的表达变化与产生绿白嵌合叶片的表型密切相关。
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| 关 键 词： | 双向电泳分析 鸢尾 绿白嵌合叶片 蛋白质 |
Two-Dimensional Polyacrylamide Gel Electrophoresis Analysis of Proteins from Albino-Green Chimeric Leaves of Iris japonica |
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| HU Jin Yong,ZENG Ying ,SANG Yu Ying.Two-Dimensional Polyacrylamide Gel Electrophoresis Analysis of Proteins from Albino-Green Chimeric Leaves of Iris japonica[J].Acta Botanica Yunnanica,2002,24(3):387-391. |
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| Authors: | HU Jin Yong ZENG Ying SANG Yu Ying |
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| Institution: | HU Jin Yong,ZENG Ying **,SANG Yu Ying |
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| Abstract: | Proteins extracted from albino green chimeric leaves of Iris japonica were separated by two dimensional polyacrylamide gel electrophoresis and relative molecular weights and isoelectric points were determined.Approximately 400 protein spots were identified in each 2-D PAGE gel.Altered expression was apparent for at least 13 proteins.The results showed that protein expression patterns changed obviously in the green and white leaf strips.In comparison with the 2-D PAGE map from Arabidopsis thaliana in SWISS-2DPAGE database, it revealed that the expression pattern of large subunit of RuBisCO and the proteins labeled W and T was closely related to the mechanism involved in chimeric variations of Iris japonic . |
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| Keywords: | Two dimensional polyacrylamide gel electrophoresis Albino green chimeric leaf Iris japonica |
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