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小桐子的组织培养和植株再生
引用本文:秦虹,宋松泉,龙春林,程红焱.小桐子的组织培养和植株再生[J].云南植物研究,2006,28(6):649-652.
作者姓名:秦虹  宋松泉  龙春林  程红焱
作者单位:1. 中国科学院西双版纳热带植物园,云南,勐腊,666303;云南农业大学园林园艺学院,云南,昆明,650201
2. 中国科学院西双版纳热带植物园,云南,勐腊,666303
3. 中国科学院昆明植物研究所,云南,昆明,650204
4. 中国科学院植物研究所,北京,100093
基金项目:国家发展改革委员会科研基金;中国科学院知识创新工程项目;中国科学院"百人计划";科技部科技基础条件平台建设计划
摘    要:以小桐子(Jatropha curcas)的胚芽、子叶、下胚轴、叶柄、叶片和茎段作为外植体,用不同浓度的6-苄基腺嘌呤(6-BA)和α-萘乙酸(NAA)对其进行愈伤组织的诱导和植株再生的研究。结果表明:在MS培养基中加入5.0mg/L6-BA和1.0mg/LNAA对愈伤组织的诱导效果最好;加入5.0mg/L6-BA和0.1mg/LNAA对不定芽的诱导最为有效,加入0.1mg/L6-BA和1.0mg/LNAA有利于芽的生长;加入1.0mg/LNAA的1/2MS培养基对生根最为有利。

关 键 词:小桐子  种子  外植体  组织培养  愈伤组织
文章编号:0253-2700(2006)06-649-04
收稿时间:2006-07-12
修稿时间:2006-08-30

Tissue Culture and Plant Regeneration of Jatropha curcas ( Euphorbiaceae )
QIN Hong,SONG Song-Quan,Long Chun-Lin,CHENG Hong-Yan.Tissue Culture and Plant Regeneration of Jatropha curcas ( Euphorbiaceae )[J].Acta Botanica Yunnanica,2006,28(6):649-652.
Authors:QIN Hong  SONG Song-Quan  Long Chun-Lin  CHENG Hong-Yan
Institution:1 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China; 2 College of Landscape and Gardening, Yunnan Agricultural University, Kunming 650201, China; 3 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; 4 Institute of Botany, ChineseAcademy of Sciences, Beijing 100093, China
Abstract:In this paper,plumules,cotyledons,hypocotyls,leaf blades,petioles and stalks of physic nut (Jatropha curcas L.) were used as explants,and callus induction and plant regeneration were studied on MS medium contained different concentrations of 6-BA and NAA.The results showed that the MS medium with 5.0?mg/L BA and 1.0?mg/L NAA was the best for callus induction,and with 5.0?mg/L BA and 0.1?mg/L NAA,for formation of adventitious buds,and with 1.0?mg/L BA and 1.0?mg/L NAA,for bud growth,and that 1/2 MS medium with 1.0?mg/L NAA was the best for formation and growth of adventitious roots.
Keywords:Callus  Explant  Jatropha curcas L    Seed  Tissue culture
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