| 黑子南瓜甘油-3-磷酸酰基转移酶基因的克隆及序列分析 |
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| 引用本文： | 杨明挚,陈善娜,鄢波.黑子南瓜甘油-3-磷酸酰基转移酶基因的克隆及序列分析[J].云南植物研究,1999,21(2):139-143. |
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| 作者姓名： | 杨明挚 陈善娜 鄢波 |
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| 作者单位： | 云南大学生物系，云南省农科院生物技术研究所 |
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| 基金项目： | 国家自然科学基金,云南省自然科学基金 |
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| 摘 要： | 依据国外报道的南瓜甘油－３－磷酸转酰酶（ＧＰＡＴ）基因的ｃＤＮＡ序列合成相应引物，用ＲＴ－ＰＣＲ技术，成功地分离了黑子南瓜（Ｃｕｃｕｒｂｉｔａｆｉｃｉｆｏｌｉａ）ＧＰＡＴ基因的ｃＤＮＡ片段，并亚克隆到了ｐＧＥＭ－Ｔ载体系统的多克隆位点上，序列分析表明黑子南瓜ＧＰＡＴ基因的ｃＤＮＡ序列及递推的氨基酸序列与南瓜（Ｃｕｃｕｒｂｉｔａｍｏｓｃｈａｔａ）相比分别具有９８％和９６５％的同源性。在１１８８ｂｐ中有２２个核苷酸发生变化，导致１３个氨基酸的改变
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| 关 键 词： | 黑子南瓜，甘油－3－磷酸酰基转移酶，cDNA |
| 修稿时间： | 1998-10-12 |
Cloning and Nucleotide
Sequence of cDNA for the Glycerol-3-
Phosphate Acyltransferase from Cucurbita ficifolia |
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| YANG Ming-Zhi ,CHEN Shan-Na ,YAN Bo ,LIU Ji-Mei ,HUANG Xing-Qi.Cloning and Nucleotide
Sequence of cDNA for the Glycerol-3-
Phosphate Acyltransferase from Cucurbita ficifolia[J].Acta Botanica Yunnanica,1999,21(2):139-143. |
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| Authors: | YANG Ming-Zhi CHEN Shan-Na YAN Bo LIU Ji-Mei HUANG Xing-Qi |
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| Institution: | YANG Ming-Zhi 1,CHEN Shan-Na 1,YAN Bo 2,LIU Ji-Mei 1,HUANG Xing-Qi 2 |
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| Abstract: | According to the cDNA sequence of GPAT from squash( Cucurbita moschata )reported in 1988,using RT-PCR technical, we isolated the cDNA encoding a glycero1-3-phosphate acyltransferase(GPAT)from figleaf gourd( Cucurbita ficifolia )cotyledons and subcloned in pGEM-T vector system. The cDNA and the deduced amino acid sequence of figleaf gourd GPAT have 98%and 96 5% homologous with squash respectively. Among the deduced amino acid residues thirteen of them are different from that of squash. |
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| Keywords: | Cucurbita ficifolia Glycerol -3-phosphate acyltransferase cDNA |
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