抽薹期叶绿素缺乏油菜突变体类囊体膜的研究

以抽薹期野生型油菜和黄化突变体叶片为材料，分析了叶片和类囊体膜的不合色素组成、色素与蛋白相对含量；比较了类囊体膜光谱特性（室温吸收光谱、荧光光谱和圆二色谱）；用温和电泳、ＳＤＳ－ＰＡＧＥ分析了类囊体色素蛋白和多肽组成。结果显示：与野生型相比，突变体叶片的蛋白质含量不变，而Ｃhaa和Ｃhlb的含量均减少；突变体类囊体膜的Ｃhla/Chlb比值较高，Ｃhl/蛋白质比值较低，ＬＨＣⅡ色素蛋白复合物的单体和三聚体含量明显减少。突变体的天线系统相对较小、捕光效率较低。

Study on the Thylakoid Membranes from A Chlorophyll-Deficient Oilseed Rape Mutant at the Bolting Stage

A comparative study on leaf and thylakoid membranes was made between a new oilse ed rape (Brassica napus) mutant Cr3529 and its wild type 3529 at the bolting stage. As compared with the wild type, Cr3529 possessed a lower content of chlo rophyll. The chlorophyll a/b ratio in the mutant was about 2-fold higher of that in the wild type. SDS-PAGE analysis showed that the content of the apoproteins of LHC II greatly declined in the mutant thylakoid membranes. The results from m ild electrophoresis and two-dimensional electrophoresis showed that the trimer L HCII (LHCII*) of mutant thylakoid membranes decreased. The room temperature abso rpation spectra, fluorescence spectra and cicular dichroism spectra indicated th at the mutant has lower efficiency of light-harvesting capacity and the size of antenna system in the mutant Cr3529 is smaller than that in wild type 3529.