重要藏药川西獐牙菜种子萌发的研究

比较了温度、生长素(赤霉素GA3)和储藏条件对川西獐牙菜(SwertiamussotiiFranch)高海拔野生自然种群和低海拔栽培后种子发芽率的影响。未经任何处理的高海拔野生自然种群种子的发芽率明显高于低海拔栽培种群。无论是赤霉素处理还是低温处理对种子的发芽率都有显著提高。经过处理后,两种来源的种子最终发芽率没有明显的变化。结果表明:(i)野生的或栽培的川西獐牙菜种子都存在休眠现象;(ii)通过引种栽培不能打破川西獐牙菜种子的休眠,该机制可能是受遗传因素的控制;(iii)赤霉素处理和4℃低温冷藏对打破种子的休眠具有重要作用。

Seed Germination of Swertia mussotii,an Important Application in Tibetan Folk Medicine

In this study,the effects of temperature,the growth regulator GA3 and storage conditions on seed germination of the biennial Swertia mussotii Franch were compared in seeds from a natural high altitude alpine site and after one-cycle of artificial cultivation at a low altitude.The untreated seeds from high altitude displayed higher germination than those from low altitude.Both GA3 and storage conditions enhanced germination from all sources.After treatment,the final germination of seeds from different sources shows no distinct difference.These results suggest that:(i) all seeds of this species,irrespective of their sources,have similar kinds of dormancy;(ii) the dormancy of this species can not been broken through one-cycle adaptation in ex situ cultivation and thus might be genetically controlled;(iii) both GA3 and 4℃ cold storage are effective for dormancy breaking in ex situ cultivation of this important medicinal species.