| 播娘蒿下胚轴高频率再生植株因素的研究 |
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| 引用本文： | 周颂东,罗鹏.播娘蒿下胚轴高频率再生植株因素的研究[J].云南植物研究,2002,24(4):515-520. |
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| 作者姓名： | 周颂东 罗鹏 |
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| 作者单位： | 1. 四川师范学院生物系,四川,南充,637002
2. 四川大学生命科学学院植物研究所,四川,成都,610064 |
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| 基金项目： | 国家自然科学基金资助项目 (396 70 0 84 ) |
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| 摘 要： | 以播娘蒿（Descurainia sophia)下胚轴为外植体，研究影响其分化的若干因素。结果表明：用附加2，4－D的培养基，对下胚轴切段预培养5d可提高分化频率；1.7mg/L AgNO3对分化出芽有很大促进作用，最佳激素组合为2.0mg/L 6-BA 0.2mg/L NAA；黄化苗的下胚轴比绿苗的下胚轴对激素反应更敏感，最高分化频率可达86.05%。再生芽转至1／2MS 0.5mg/L NAA 0.5mg/L IBA的培养基中，生根率100％。
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| 关 键 词： | 播娘蒿 下胚轴 高频率再生植株 影响因素 |
| 文章编号： | 0253-2700(2002)04-0515-06 |
| 修稿时间： | 2001年8月20日 |
Studies on Factor of High Rate Regeneration Plant of Descurainia sophia Hypocotyl |
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| ZHOU Song Dong ,LUO Peng.Studies on Factor of High Rate Regeneration Plant of Descurainia sophia Hypocotyl[J].Acta Botanica Yunnanica,2002,24(4):515-520. |
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| Authors: | ZHOU Song Dong LUO Peng |
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| Institution: | ZHOU Song Dong 1,LUO Peng 2 |
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| Abstract: | The hypocotyl of D sophia was cultured to study the effect of some factors on the rate of bud regeneration.The results showed that the rate of hypocotyl differentiating was improved via a preculture of five day period with the medium containing 2,4-D;AgNO 3(1 7?mg/L) could greatly improve the differentiating rate of hypocotyl;the best hormone combination was 2 0?mg/L 6-BA+0 2?mg/L NAA when 1 7?mg/L AgNO 3 was added to this medium;the hypocotyl of yellow seedlings was more sensitive to hormone than that of green seedlings,its differentiating rate could reach as high as 86 05%?When the regeneration buds were transferred 1/2 MS+0 5?mg/L NAA+0 5?mg/L IBA,the rate of regeneration roots reached 100%. |
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| Keywords: | Descurainia sophia Hypocotyl Regeneration plant Influential factors |
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