多叶重楼遗传多样性的ISSR分析

采用ISSR分子标记对多叶重楼种下3个变种的8个居群共208份样品的遗传多样性进行了分析。l4条引物共检测到251个清晰的扩增位点,其中多态位点235个。在物种水平上,多态位点百分率(PPB)达93.63%,Nei′s基因多样性指数(HE)为0.2204、Shannon′s信息指数(HO)为0.3532。在居群水平上,多态位点百分率(PPB)为50.45%,Nei′s基因多样性指数(HE)为0.1405,Shannon′s信息指数(HO)为0.2194,这些均表明多叶重楼的遗传多样性水平较高。此外,还用NTSYS软件对样品进行了UPGMA聚类分析,结果显示滇重楼的6个居群聚为一支,滇重楼与狭叶重楼有较近的亲缘关系,而与长药隔重楼之间的遗传分化较大。本研究提出了合理保护重楼植物资源的若干措施,为进一步开展滇重楼优质种质引种驯化,实现种质资源多样性的合理保护和可持续利用提供参考。

Assessment of Genetic Diversity of Paris polyphylla (Trilliaceae) by ISSR Markers

Some species of the genus Paris are of high medicinal value.Severe human activities particularly over-harvesting have led to recent declines of their population size and fragmented habitat patches.Inter-simple sequence repeat markers(ISSR) were applied to investigate the genetic diversity of eight populations of Paris polyphylla in Yunnan and Sichuan Provinces.Fourteen primers resulted to a total of 251 bands from the eight populations,of which 235 were polymorphic,which showes a high level of genetic diversity in this species.A relatively high level of genetic diversity was revealed:PPB=93.63%,HE=0.2204 and HO=0.3532 at the species level;PPB=50.45%,HE=0.1405 and HO=0.2194 at the population level.The GST=0.3625 indicated a high degree of genetic differentiation among populations.Genetic variation occurred mainly within populations.In UPGMA cluster analysis the six populations of P.polyphylla var.yunnanensis were divided into two clades,and P.polyphylla var.yunnanensis is more related to P.polyphylla var.stenophylla than to P.polyphylla var.pseudothibetica.In this paper,some conservation concerns were discussed which would be helpful for the conservation and sustainable utilization of this medicine resources.