云南山楂中吲哚乙酸、脱落酸、玉米素和玉米素核苷的内源水平及其生根率

以云南山楂(Crataegus scabrifolia (Franch.)Rchd.)几个株系的成年树和实生苗的离体培养芽条为材料,用酶联免疫吸附测定法(ELISA)测定了它们的叶片中吲哚乙酸(IAA),脱落酸(ABA),玉米素和玉米素核苷(Z+ZR)的内源水平,探讨了这三种激素及其之间的平衡与生根率的相关性。结果发现:1.IAA内源水平之差异存在于株系间。2.内源(Z+ZR)在株系间无显著差异,而幼树发育至成年树的过程中,与IAA的平衡是多样的,同成年树的生根率似有以下相关性,当IAA水平降低,同时(Z+ZR)水平升高,有利于生根,反之则抑制生根。3.ABA内源水平无论成年或幼年树都呈低水平且无显著差异。

THE ENDOGENOUS LEVELS OF INDOLE-3-ACETIC ACID, ABSCISIC ACID, AND ZEATIN PLUS ZEATIN-RIBOSIDE IN CULTURAL SHOOTS OF CRATEGUS SCABRIFOLIA AND THEIR ROOTING RATIO

Cultural shoots derived from adult and their seedings plant of C.scabrifolia were used as experimental material.Endogenous levels of IAA, ABA and Z+ZR were determined by methed of enzymelinked immunosorbend assay(ELISA). We also probed into relation among the plant hormones mentioned above and different rooting ratio in cultured shoots derived from seedings and adult plants of C.scabrifolia. The experimental results show: The endogenous level of IAA depends upon individual line. The balance of IAA and Z+ZR during the course from seeding to adult plant is multiform, when endogenous levels of IAA increase and Z+ZR decrease, it is of benefit to induce root in cultural shoots derived from abult plant. Conversely, it is dis-advanta-geous to induce root.The endogenous level of ABA in all shoots is at lower level, and no remarkable difference between the one derived from seedings or adult plant was observed.