Proton leak modulation in testicular mitochondria affects reactive oxygen species production and lipid peroxidation |
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Authors: | Ana Sofia Rodrigues Beatriz Lacerda António J M Moreno João Ramalho‐Santos |
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Institution: | 1. Center for Neuroscience and Cell Biology, Department of Life Sciences, University of Coimbra, Coimbra 3004‐517, Portugal;2. Department of Life Sciences, Institute of Marine Research, University of Coimbra, Coimbra, Portugal |
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Abstract: | Mitochondrial proton leak can account for almost 20% of oxygen consumption and it is generally accepted that this process contributes to basal metabolism. In order to clarify the role of basal proton leak in testicular mitochondria, we performed a comparative study with kidney and liver mitochondrial fractions. Proton leak stimulated by linoleic acid and inhibited by guanosine diphosphate (GDP) was detected, in a manner that was correlated with protein levels for uncoupling protein 2 (UCP2) in the three fractions. Modulation of proton leak had an effect on reactive oxygen species production as well as on lipid peroxidation, and this effect was also tissue‐dependent. However, a possible role for the adenine nucleotide transporter (ANT) in testicular mitochondria proton leak could not be excluded. The modulation of proton leak appears as a possible and attractive target to control oxidative stress with implications for male gametogenesis. Copyright © 2010 John Wiley & Sons, Ltd. |
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Keywords: | mitochondria ROS testis reproduction proton leak |
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