The mechanism of placental alkaline phosphatase induction in vitro |
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| Authors: | S Nozawa S Narisawa R Iizuka T Fukasawa T Kohji P K Nakane K Hirano J L Millan |
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| Institution: | Department of Obstetrics and Gynecology, Keio University School of Medicine, Japan. |
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| Abstract: | The mechanism of placental alkaline phosphatase (PLAP) induction by prednisolone in a uterine cervical epidermoid cancer cell line SKG-IIIa was investigated in vitro by enzyme-cytochemistry, enzyme immunoassay, Northern and Southern blot analysis, and in situ hybridization. Enzyme-cytochemical alkaline phosphatase (ALP) staining and immunoassay revealed increased levels of PLAP (heat-stable ALP) in prednisolone-treated cells. Northern blot analysis and in situ hybridization showed increased amounts of PLAP mRNA. Southern blot analysis indicated that PLAP was not a product of an amplified or rearranged gene. These findings suggest that the induction of PLAP mRNA in SKG-IIIa cells by prednisolone in turn increased the levels of PLAP. |
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| Keywords: | Placental alkaline phosphatase prednisolone induction PLAP cDMA Northern and Southern blotting in situ hybridization |
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