Comparative genomic assessment of Multi-Locus Sequence Typing: rapid accumulation of genomic heterogeneity among clonal isolates of <Emphasis Type="Italic">Campylobacter jejuni</Emphasis> |
| |
| Authors: | Eduardo N Taboada Joanne M MacKinnon Christian C Luebbert Victor PJ Gannon John HE Nash Kris Rahn |
| |
| Institution: | (1) Laboratory for Foodborne Zoonoses (Lethbridge Unit), Public Health Agency of Canada c/o Animal Diseases Research Institute, PO Box 640, Township Road 9-1, Lethbridge, Alberta, T1J 3Z4, Canada;(2) Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, 110 Stone Road West, Guelph, Ontario, N1G 3W4, Canada;(3) Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario, K1A 0R6, Canada |
| |
| Abstract: | Background Multi-Locus Sequence Typing (MLST) has emerged as a leading molecular typing method owing to its high ability to discriminate
among bacterial isolates, the relative ease with which data acquisition and analysis can be standardized, and the high portability
of the resulting sequence data. While MLST has been successfully applied to the study of the population structure for a number
of different bacterial species, it has also provided compelling evidence for high rates of recombination in some species.
We have analyzed a set of Campylobacter jejuni strains using MLST and Comparative Genomic Hybridization (CGH) on a full-genome microarray in order to determine whether
recombination and high levels of genomic mosaicism adversely affect the inference of strain relationships based on the analysis
of a restricted number of genetic loci. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
