Analysis of the developmental expression of small VCP-interacting protein and its interaction with steroidogenic acute regulatory protein in Leydig cells |
| |
| Institution: | 1. Department of Histology and Embryology, Istanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Istanbul, 34098, Turkey;2. Department of Biochemistry, Istanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Istanbul, 34098, Turkey;1. Constantine the Philosopher University, 949 74, Nitra, Slovakia;2. Research Institute of Animal Production, National Agricultural and Food Center, 951 41 Lu?ianky, Slovakia;3. King Saud University, College of Science, Zoology Department, 11451, Riyadh, Saudi Arabia;1. Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Selangor, Malaysia;2. I-PerFForm, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Selangor, Malaysia;1. Departments of Zoology, Faculty of Science, Damietta University, Egypt;2. Departments of Botany & Microbiology, Faculty of Science, Damietta University, Egypt;1. University of Veterinary Medicine, Department of Obstetrics and Reproduction, István str 2., H-1078 Budapest, Hungary;2. Department of Anatomy, University of Pecs Medical School, MTA-PTE PACAP Research Team, Szigeti str 12., H-7624 Pécs, Hungary |
| |
| Abstract: | Small VCP-interacting protein (SVIP) is a 9-kDa protein that is composed of 76 amino acids, and it plays a role in the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Recent studies have shown that SVIP is an androgen-responsive protein and its expression is regulated by androgens. Because no data are available regarding the cellular localization and expression of SVIP in the mouse testis, where androgens are highly expressed, immunohistochemistry and western blotting were performed. In the fetal testis, we found that moderate but consistent staining of SVIP is present in the cytoplasm of Leydig cells. In prepubertal and adult life, SVIP remains present in Leydig cells as well as in the cytoplasm of some peritubular and Sertoli cells. From postnatal day 15 onward, SVIP is strongly expressed in the cytoplasm of Leydig cells.Furthermore, TM3, MA-10 Leydig and Sertoli cell lines were also used to evaluate the expression of SVIP. To identify the interacting partners, such as steroidogenic acute regulatory (STAR) protein, colocalization studies were performed by fluorescence microscopy, showing that STAR colocalized with SVIP in the adult mouse testis. The expression changes of STAR were studied by using SVIP siRNAs in Leydig cell line cultures. Depletion of SVIP resulted in decreased expression of STAR. Additionally, the number and size of lipid droplets were significantly increased in SVIP-depleted Leydig cells. Taken together, our data identify SVIP as a marker of Leydig cell lineage and as a regulator of STAR protein expression and lipid droplet status in Leydig cells. |
| |
| Keywords: | Small VCP-interacting protein (SVIP) Steroidogenic acute regulator protein (STAR) Testis Leydig cells TM3 MA-10 15P1 |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
