An autoclavable glucose biosensor for microbial fermentation monitoring and control |
| |
Authors: | Phelps M R Hobbs J B Kilburn D G Turner R F |
| |
Institution: | Biotechnology Laboratory, The University of British Columbia, 237-6174 University Boulevard, Vancouver, British Columbia V6T 1Z3, Canada. |
| |
Abstract: | The design, construction, and characterization of a prototype-regenerable glucose biosensor based on the reversible immobilization of glucose oxidase (GOx) using cellulose binding domain (CBD) technology is described. GOx, chemically linked to CBD, is immobilized by binding to a cellulose matrix on the sensor-indicating electode. Enzyme immobilization can be reversed by perfusing the cellulose matrix with a suitable eluting solution. An autocavable sensor membrane system is employed which is shown to be practical for use in real microbial fermentations. The prototype glucose biosensor was used without failure or deterioration during fed-batch fermentations of Escherichia coli reaching a maximum cell density of 85 g (dry weight)/L. Medium glucose concentration based on sensor output correlated closely with off-line glucose analysis and was controlled manually at 0.44 +/- 0.2 g/L for 2 h based on glucose sensor output. The sensor enzyme component could be eluted and replaced without interrupting the fermentation. To our knowledge, no other in situ biosensor has been used for such an extended period of time in such a high-cell-density fermentation. (c) 1995 John Wiley & Sons, Inc. |
| |
Keywords: | glucose biosensor biosensor glucose oxidase cellulose binding domain (CBD) fermentation monitoring |
本文献已被 PubMed 等数据库收录! |
|